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dc.contributor.authorHOANG, PHUONG
dc.contributor.authorKhashab, Niveen M.
dc.date.accessioned2019-10-17T13:28:26Z
dc.date.available2019-10-17T13:28:26Z
dc.date.issued2019-10-03
dc.identifier.citationHoang, P., & Khashab, N. M. (2019). Barcoding Amino Acids for Mutation Screening in Amyloid Beta Peptides. Small Methods, 1900611. doi:10.1002/smtd.201900611
dc.identifier.doi10.1002/smtd.201900611
dc.identifier.urihttp://hdl.handle.net/10754/658657
dc.description.abstractAmino acid (AA) substitutions are directly correlated with specific pathologies such as Alzheimer's disease, making their rapid screening and detection critical to treatment and scientific study. A proof-of-concept implementation of the label-free and noninvasive Raman spectroscopy technique for the detection of AA substitutions in primary peptide fragments is demonstrated. By encoding the Raman “fingerprint” of individual AAs into binary formats called optical identification tags (OITs), a library of identifiers is created, which can then be used for detecting mutations. When the recorded Raman signal is enhanced by using surface-enhanced Raman scattering substrate, the mutation screening strategy can detect a single point missense mutation in an 11-AA peptide fragment of amyloid beta Aβ(25–35) and a frameshift mutation in a 42-AA fragment Aβ(1–42) down to picomolar concentrations. The combination of high sensitivity and simple operation makes the use of OITs a promising approach for high-throughput automated screening.
dc.description.sponsorshipThis work was supported by King Abdullah University of Science and Technology (KAUST). The authors gratefully acknowledge useful conversations with Dr. Emily Ringe. The authors also acknowledge Dr. Jeremy A. Bau for proofreading the manuscript. They also thank Ivan Gromicho, a scientific illustrator at KAUST, for creating Figure 1 and ToC image.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/smtd.201900611
dc.rightsArchived with thanks to Small Methods
dc.subjectcomposition screening
dc.subjectencoded libraries
dc.subjectmutation points
dc.subjectoptical identification tags (OIT)
dc.subjectsurface-enhanced Raman spectroscopy (SERS)
dc.titleBarcoding Amino Acids for Mutation Screening in Amyloid Beta Peptides
dc.typeArticle
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Center
dc.contributor.departmentChemical Engineering Program
dc.contributor.departmentChemical Science Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentSmart Hybrid Materials (SHMs) lab
dc.identifier.journalSmall Methods
dc.eprint.versionPost-print
kaust.personHoang, Phuong Mai
kaust.personKhashab, Niveen M.
kaust.acknowledged.supportUnitscientific illustrator


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