Disruption of the coordination between host circadian rhythms and malaria parasite development alters the duration of the intraerythrocytic cycle
Type
PreprintAuthors
Subudhi, Amit
O'Donnell, Aidan John

Ramaprasad, Abhinay

Abkallo, Hussein M.

Kaushik, Abhinav

Ansari, Hifzur Rahman

Abdel-Haleem, Alyaa M.

Rached, Fathia Ben

Kaneko, Osamu

Culleton, Richard

Reece, Sarah E.

Pain, Arnab

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Pathogen Genomics Group
KAUST Grant Number
BAS/1/1020-01-01Date
2019-10-03Permanent link to this record
http://hdl.handle.net/10754/656930
Metadata
Show full item recordAbstract
Malaria parasites complete their intra-erythrocytic developmental cycle (IDC) in multiples of 24 hours (depending on the species), suggesting a circadian basis to the asexual cell cycle, but the mechanism controlling this periodicity is unknown. Combining in vivo and in vitro approaches using rodent and human malaria parasites, we reveal that: (i) 57% of Plasmodium chabaudi genes exhibit 24 h circadian periodicity in transcription; (ii) 58% of these genes lose transcriptional rhythmicity when the IDC is out-of-synchrony with host rhythms; (iii) 9% of Plasmodium falciparum genes show circadian transcription under free-running conditions; (iv) Serpentine receptor 10 (SR10) has a circadian transcription profile and disrupting it in rodent malaria parasites shortens the IDC by 2-3 hours; (v) Multiple processes including DNA replication and the ubiquitin and proteasome pathways are affected by loss of coordination with host rhythms and by disruption of SR10. Our results show that malaria parasites are at least partly responsible for scheduling their IDCs explaining the fitness benefits of coordination with host rhythms.Citation
Subudhi, A. K., O’Donnell, A. J., Ramaprasad, A., Abkallo, H. M., Kaushik, A., Ansari, H. R., … Pain, A. (2019). Disruption of the coordination between host circadian rhythms and malaria parasite development alters the duration of the intraerythrocytic cycle. doi:10.1101/791046Sponsors
The project was supported by a faculty baseline funding (BAS/1/1020-01-01) from the King Abdullah University of Science and Technology (KAUST) to AP. RC is supported by Japanese Society for the Promotion of Science (JSPS), Japan Grant-in-Aid for Scientific Research Nos. 24255009, 25870525, 16K21233 and 19K07526. SER and AJOD are supported by Wellcome (202769/Z/16/Z; 204511/Z/16/Z), the Royal Society (UF110155; NF140517) and the Human Frontier Science Program (RGP0046/2013). The authors thank the staff of the Bioscience Core Laboratory in KAUST for sequencing RNAseq libraries and all members of the Reece lab at the University of Edinburgh and pathogen genomics lab at KAUST for assistance during the experiments. This work was partly conducted at the Joint Usage / Research Center for Tropical Disease, Institute of Tropical Medicine, Nagasaki University, Japan.Publisher
Cold Spring Harbor LaboratoryDOI
10.1101/791046Additional Links
http://biorxiv.org/lookup/doi/10.1101/791046ae974a485f413a2113503eed53cd6c53
10.1101/791046
Scopus Count
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