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dc.contributor.authorAlvarez, Juan
dc.contributor.authorSaudino, Giovanni
dc.contributor.authorMusteata, Valentina-Elena
dc.contributor.authorMadhavan, Poornima
dc.contributor.authorGenovese, Alessandro
dc.contributor.authorBehzad, Ali Reza
dc.contributor.authorSougrat, Rachid
dc.contributor.authorBoi, Cristiana
dc.contributor.authorPeinemann, Klaus-Viktor
dc.contributor.authorNunes, Suzana Pereira
dc.date.accessioned2019-10-07T08:26:15Z
dc.date.available2019-10-07T08:26:15Z
dc.date.issued2019-09-27
dc.identifier.citationAlvarez, J., Saudino, G., Musteata, V., Madhavan, P., Genovese, A., Behzad, A. R., … Nunes, S. P. (2019). 3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods. Scientific Reports, 9(1). doi:10.1038/s41598-019-50338-2
dc.identifier.doi10.1038/s41598-019-50338-2
dc.identifier.urihttp://hdl.handle.net/10754/656929
dc.description.abstractHighly porous particles with internal triply periodic minimal surfaces were investigated for sorption of proteins. The visualization of the complex ordered morphology requires complementary advanced methods of electron microscopy for 3D imaging, instead of a simple 2D projection: transmission electron microscopy (TEM) tomography, slice-and-view focused ion beam (FIB) and serial block face (SBF) scanning electron microscopy (SEM). The capability of each method of 3D image reconstruction was demonstrated and their potential of application to other synthetic polymeric systems was discussed. TEM has high resolution for details even smaller than 1 nm, but the imaged volume is relatively restricted (2.5 μm)3. The samples are pre-sliced in an ultramicrotome. FIB and SBF are coupled to a SEM. The sample sectioning is done in situ, respectively by an ion beam or an ultramicrotome, SBF, a method so far mostly applied only to biological systems, was particularly highly informative to reproduce the ordered morphology of block copolymer particles with 32-54 nm nanopores and sampling volume (20 μm)3.
dc.description.sponsorshipThe work was supported by the King Abdullah University of Science and Technology (KAUST), base line funding BAS/1/1057-01-01. The authors thank LNLS (Brazil) for access to the SAXS1 beamline, particularly F. Meneau.
dc.publisherSpringer Science and Business Media LLC
dc.relation.urlhttp://www.nature.com/articles/s41598-019-50338-2
dc.rightsArchived with thanks to Scientific Reports
dc.title3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods
dc.typeArticle
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Center
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentChemical Engineering Program
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentNanostructured Polymeric Membrane Lab
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionAlma Mater Studiorum-Università di Bologna, via Terracini 28, Bologna, 40131, Italy
kaust.personAlvarez, Juan
kaust.personSaudino, Giovanni
kaust.personMusteata, Valentina-Elena
kaust.personMadhavan, Poornima
kaust.personGenovese, Alessandro
kaust.personBehzad, Ali Reza
kaust.personSougrat, Rachid
kaust.personPeinemann, Klaus-Viktor
kaust.personNunes, Suzana Pereira
kaust.grant.numberBAS/1/1057-01-01
refterms.dateFOA2019-10-07T08:27:04Z
dc.date.published-online2019-09-27
dc.date.published-print2019-12


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