3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods
Behzad, Ali Reza
Nunes, Suzana Pereira
KAUST DepartmentKing Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), Advanced Membranes and Porous Materials Center, Thuwal, 23955-6900, Saudi Arabia
Biological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Chemical and Biological Engineering Program
Advanced Membranes and Porous Materials Research Center
Physical Sciences and Engineering (PSE) Division
KAUST Grant NumberBAS/1/1057-01-01
Permanent link to this recordhttp://hdl.handle.net/10754/656929
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AbstractHighly porous particles with internal triply periodic minimal surfaces were investigated for sorption of proteins. The visualization of the complex ordered morphology requires complementary advanced methods of electron microscopy for 3D imaging, instead of a simple 2D projection: transmission electron microscopy (TEM) tomography, slice-and-view focused ion beam (FIB) and serial block face (SBF) scanning electron microscopy (SEM). The capability of each method of 3D image reconstruction was demonstrated and their potential of application to other synthetic polymeric systems was discussed. TEM has high resolution for details even smaller than 1 nm, but the imaged volume is relatively restricted (2.5 μm)3. The samples are pre-sliced in an ultramicrotome. FIB and SBF are coupled to a SEM. The sample sectioning is done in situ, respectively by an ion beam or an ultramicrotome, SBF, a method so far mostly applied only to biological systems, was particularly highly informative to reproduce the ordered morphology of block copolymer particles with 32-54 nm nanopores and sampling volume (20 μm)3.
CitationAlvarez, J., Saudino, G., Musteata, V., Madhavan, P., Genovese, A., Behzad, A. R., … Nunes, S. P. (2019). 3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods. Scientific Reports, 9(1). doi:10.1038/s41598-019-50338-2
SponsorsThe work was supported by the King Abdullah University of Science and Technology (KAUST), base line funding BAS/1/1057-01-01. The authors thank LNLS (Brazil) for access to the SAXS1 beamline, particularly F. Meneau.
PublisherSpringer Science and Business Media LLC