• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    s41598-019-50338-2.pdf
    Size:
    2.364Mb
    Format:
    PDF
    Description:
    publisher's version
    Download
    Type
    Article
    Authors
    Alvarez, Juan
    Saudino, Giovanni
    Musteata, Valentina-Elena
    Madhavan, Poornima cc
    Genovese, Alessandro
    Behzad, Ali Reza
    Sougrat, Rachid
    Boi, Cristiana
    Peinemann, Klaus-Viktor cc
    Nunes, Suzana Pereira cc
    KAUST Department
    Advanced Membranes and Porous Materials Research Center
    Biological and Environmental Sciences and Engineering (BESE) Division
    Chemical Engineering Program
    Electron Microscopy
    Environmental Science and Engineering Program
    Nanostructured Polymeric Membrane Lab
    Physical Science and Engineering (PSE) Division
    KAUST Grant Number
    BAS/1/1057-01-01
    Date
    2019-09-27
    Online Publication Date
    2019-09-27
    Print Publication Date
    2019-12
    Permanent link to this record
    http://hdl.handle.net/10754/656929
    
    Metadata
    Show full item record
    Abstract
    Highly porous particles with internal triply periodic minimal surfaces were investigated for sorption of proteins. The visualization of the complex ordered morphology requires complementary advanced methods of electron microscopy for 3D imaging, instead of a simple 2D projection: transmission electron microscopy (TEM) tomography, slice-and-view focused ion beam (FIB) and serial block face (SBF) scanning electron microscopy (SEM). The capability of each method of 3D image reconstruction was demonstrated and their potential of application to other synthetic polymeric systems was discussed. TEM has high resolution for details even smaller than 1 nm, but the imaged volume is relatively restricted (2.5 μm)3. The samples are pre-sliced in an ultramicrotome. FIB and SBF are coupled to a SEM. The sample sectioning is done in situ, respectively by an ion beam or an ultramicrotome, SBF, a method so far mostly applied only to biological systems, was particularly highly informative to reproduce the ordered morphology of block copolymer particles with 32-54 nm nanopores and sampling volume (20 μm)3.
    Citation
    Alvarez, J., Saudino, G., Musteata, V., Madhavan, P., Genovese, A., Behzad, A. R., … Nunes, S. P. (2019). 3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods. Scientific Reports, 9(1). doi:10.1038/s41598-019-50338-2
    Sponsors
    The work was supported by the King Abdullah University of Science and Technology (KAUST), base line funding BAS/1/1057-01-01. The authors thank LNLS (Brazil) for access to the SAXS1 beamline, particularly F. Meneau.
    Publisher
    Springer Science and Business Media LLC
    Journal
    Scientific Reports
    DOI
    10.1038/s41598-019-50338-2
    Additional Links
    http://www.nature.com/articles/s41598-019-50338-2
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41598-019-50338-2
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Advanced Membranes and Porous Materials Research Center; Environmental Science and Engineering Program; Physical Science and Engineering (PSE) Division; Chemical Engineering Program

    entitlement

     
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.