Show simple item record

dc.contributor.authorTunÇ, İlknur
dc.contributor.authorSusapto, Hepi Hari
dc.date.accessioned2019-09-10T07:44:42Z
dc.date.available2019-09-10T07:44:42Z
dc.date.issued2019-09-08
dc.identifier.doi10.1166/jnn.2020.17141
dc.identifier.urihttp://hdl.handle.net/10754/656709
dc.description.abstractSurface-enhanced Raman spectroscopy (SERS) has drawn attention in recent years for imaging biologicalmolecules as an analytical tool due to its label-free approach. The SERS approach can be used in tracking organic molecules and monitoring unique Raman spectra of the organic molecules bound to metal nanoparticles (NPs). In this paper, the molecular specifity of Raman Spectroscopy was used together with self-assembled monolayer of metallic AuNPs as a sensor platform in order to detect CA125 antibody-antigen probe molecules. Highly enhanced electromagnetic fields localized around neighboring AuNPs provide hot-spot construction due to the spatial distribution of SERS enhancement on the CA125 proteins at nM concentration level. Time resolved SERS mapping of CA125 antibody and antigen couples was recorded. Even though blinking behavior was observed for some cases, vast variety SERS signals from CA125 proteins were highly reproducible. Blinking behavior is attributed to single molecular detection. Distinguished feature of SERS mapping images of CA125 antibody and antigen with such a low concentration level is very promising for this technique to be used for diagnostic purposes.
dc.publisherAmerican Scientific Publishers
dc.relation.urlhttps://www.ingentaconnect.com/content/10.1166/jnn.2020.17141
dc.rightsArchived with thanks to Journal of nanoscience and nanotechnology
dc.subjectAntibody
dc.subjectAntigen
dc.subjectCA125
dc.subjectCancer Biomarkers
dc.subjectHot Spots
dc.subjectPlasmonic Nanoparticles
dc.subjectSERS
dc.titleLabel-Free Detection of Ovarian Cancer Antigen CA125 by Surface Enhanced Raman Scattering.
dc.typeArticle
dc.contributor.departmentBioscience Program
dc.identifier.journalJournal of nanoscience and nanotechnology
dc.eprint.versionPost-print
dc.contributor.institutionUniversity of Turkish Aeronautical Association, Turkkusu Campus, Etimesgut, 06790 Ankara, Turkey.
kaust.personSusapto, Hepi Hari


This item appears in the following Collection(s)

Show simple item record