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dc.contributor.authorButt, Haroon
dc.contributor.authorZaidi, Syed Shan-e-Ali
dc.contributor.authorHassan, Norhan
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2019-09-10T05:52:32Z
dc.date.available2019-09-10T05:52:32Z
dc.date.issued2019-09-04
dc.identifier.doi10.1016/j.tibtech.2019.08.001
dc.identifier.urihttp://hdl.handle.net/10754/656702
dc.description.abstractDirected evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0167779919302057
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Trends in biotechnology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Trends in biotechnology, [[Volume], [Issue], (2019-09-04)] DOI: 10.1016/j.tibtech.2019.08.001 . © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCRISPR-Cas9
dc.subjectCRISPR-directed evolution
dc.subjectcrop breeding
dc.subjectHDR
dc.subjectNHEJ
dc.subjecttrait engineering
dc.titleCRISPR-Based Directed Evolution for Crop Improvement.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalTrends in biotechnology
dc.rights.embargodate2020-09-04
dc.eprint.versionPost-print
dc.contributor.institutionPlant Genetics Lab, TERRA Research and Teaching Centre, Gembloux Agro BioTech, University of Liège, Passage des Déportés 2, 5030 Gembloux, Belgium.
kaust.personButt, Haroon
kaust.personHassan, Norhan
kaust.personMahfouz, Magdy M.
refterms.dateFOA2019-09-11T13:20:10Z


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