An organic electrochemical transistor integrated with a molecularly selective isoporous membrane for amyloid-β detection.
Name:
Revised Manuscript_Wustoni et al_Biosensors and Bioelectronic 2019.pdf
Size:
834.4Kb
Format:
PDF
Description:
Accepted Manuscript
Embargo End Date:
2021-08-26
Name:
1-s2.0-S0956566319306402-mmc1.docx
Size:
3.413Mb
Format:
Microsoft Word 2007
Description:
supplementary file
Embargo End Date:
2021-08-26
Type
ArticleAuthors
Wustoni, Shofarul
Wang, Shaofei

Alvarez, Juan R
Hidalgo, Tania C
Nunes, Suzana Pereira

Inal, Sahika

KAUST Department
Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.Biological and Environmental Sciences and Engineering (BESE) Division
Bioscience Program
Environmental Science and Engineering Program
Date
2019-08-05Online Publication Date
2019-08-05Print Publication Date
2019-10Embargo End Date
2021-08-26Permanent link to this record
http://hdl.handle.net/10754/656660
Metadata
Show full item recordAbstract
Alzheimer's disease (AD) is a progressive neurodegenerative disease associated with severe memory loss and impaired cognitive skills. A common pathological change found in AD-affected brains is the accumulation of a peptide named amyloid-β (Aβ) that can form plaques. Aβ aggregates are visible to structural scanning tools; however, these bulky and expensive instruments are accessible to trained personnel in clinical settings only, thus hampering timely diagnosis of the disease, particularly in low-resource settings. In this work, we design an organic electrochemical transistor (OECT) for in vitro detection of Aβ aggregates in human serum. The OECT channel is integrated with a nanostructured isoporous membrane which has a strong affinity for Aβ aggregates. The detection mechanism relies on the membrane capturing Aβ aggregates larger than the size of its pores and thus blocking the penetration of electrolyte ions into the channel underneath. Combining the high transconductance of the OECT with the precise porosity and selectivity of the membrane, the device detects the presence of Aβ aggregates in human serum samples with excellent sensitivity. This is the first-time demonstration of a biofunctionalized, nanostructured, and isoporous membrane integrated with a high-performance transistor for biosensing. This robust, low-power, non-invasive, and miniaturized sensor aids in the development of point-of-care tools for early diagnosis of AD.Citation
Wustoni, S., Wang, S., Alvarez, J. R., Hidalgo, T. C., Nunes, S. P., & Inal, S. (2019). An organic electrochemical transistor integrated with a molecularly selective isoporous membrane for amyloid-β detection. Biosensors and Bioelectronics, 143, 111561. doi:10.1016/j.bios.2019.111561Sponsors
S. W. and S. I. gratefully acknowledge financial support from the KAUST Office of Sponsored Research (OSR) under Award CRF-2019- SENSORS-2719.Publisher
Elsevier BVJournal
Biosensors & bioelectronicsAdditional Links
https://linkinghub.elsevier.com/retrieve/pii/S0956566319306402ae974a485f413a2113503eed53cd6c53
10.1016/j.bios.2019.111561