Type
ArticleAuthors
Coluccio, Maria LauraD’Attimo, Maria Antonia
Cristiani, Costanza Maria
Candeloro, Patrizio

Parrotta, Elvira
Dattola, Elisabetta
Guzzi, Francesco

Cuda, Giovanni

Lamanna, Ernesto

Carbone, Ennio
Krühne, Ulrich

Di Fabrizio, Enzo M.

Perozziello, Gerardo
KAUST Department
Material Science and Engineering ProgramPhysical Science and Engineering (PSE) Division
Date
2019-08-20Permanent link to this record
http://hdl.handle.net/10754/656611
Metadata
Show full item recordAbstract
This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.Citation
Coluccio, M. L., D’Attimo, M. A., Cristiani, C. M., Candeloro, P., Parrotta, E., Dattola, E., … Perozziello, G. (2019). A Passive Microfluidic Device for Chemotaxis Studies. Micromachines, 10(8), 551. doi:10.3390/mi10080551Sponsors
This work was supported by the project for young researchers financed from the Italian Ministry of Health “High throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University “Magna Graecia” of Catanzaro.Publisher
MDPI AGJournal
MicromachinesAdditional Links
https://www.mdpi.com/2072-666X/10/8/551ae974a485f413a2113503eed53cd6c53
10.3390/mi10080551
Scopus Count
Except where otherwise noted, this item's license is described as Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).