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dc.contributor.authorZarban, Randa Alhassan Yahya
dc.contributor.authorVogler, Malvina M.
dc.contributor.authorWong, Aloysius Tze
dc.contributor.authorEppinger, Jörg
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorGehring, Christoph A
dc.date.accessioned2019-08-18T08:49:18Z
dc.date.available2019-08-18T08:49:18Z
dc.date.issued2019-07-24
dc.identifier.citationZarban, R., Vogler, M., Wong, A., Eppinger, J., Al-Babili, S., & Gehring, C. (2019). Discovery of a Nitric Oxide-Responsive Protein in Arabidopsis thaliana. Molecules, 24(15), 2691. doi:10.3390/molecules24152691
dc.identifier.doi10.3390/molecules24152691
dc.identifier.urihttp://hdl.handle.net/10754/656458
dc.description.abstractIn plants, much like in animals, nitric oxide (NO) has been established as an important gaseous signaling molecule. However, contrary to animal systems, NO-sensitive or NO-responsive proteins that bind NO in the form of a sensor or participating in redox reactions have remained elusive. Here, we applied a search term constructed based on conserved and functionally annotated amino acids at the centers of Heme Nitric Oxide/Oxygen (H-NOX) domains in annotated and experimentally-tested gas-binding proteins from lower and higher eukaryotes, in order to identify candidate NO-binding proteins in Arabidopsis thaliana. The selection of candidate NO-binding proteins identified from the motif search was supported by structural modeling. This approach identified AtLRB3 (At4g01160), a member of the Light Response Bric-a-Brac/Tramtrack/Broad Complex (BTB) family, as a candidate NO-binding protein. AtLRB3 was heterologously expressed and purified, and then tested for NO-response. Spectroscopic data confirmed that AtLRB3 contains a histidine-ligated heme cofactor and importantly, the addition of NO to AtLRB3 yielded absorption characteristics reminiscent of canonical H-NOX proteins. Furthermore, substitution of the heme iron-coordinating histidine at the H-NOX center with a leucine strongly impaired the NO-response. Our finding therefore established AtLRB3 as a NO-interacting protein and future characterizations will focus on resolving the nature of this response.
dc.description.sponsorshipThe work was supported by King Abdullah University of Science and Technology and in particular, doctoral fellowships to R.Z. and M.V. A.W. is supported by the National Natural Science Foundation of China (Grant no. 31850410470) and the Zhejiang Provincial Natural Science Foundation of China (Grant no. LQ19C130001).
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/1420-3049/24/15/2691
dc.rightsby the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectArabidopsis thaliana
dc.subjectnitric oxide
dc.subjectHeme Nitric Oxide/Oxygen (H-NOX) domain
dc.subjectBric-a-Brac/Tramtrack/Broad Complex (BTB)
dc.subjectNO-sensitive protein
dc.titleDiscovery of a Nitric Oxide-Responsive Protein in Arabidopsis thaliana.
dc.typeArticle
dc.contributor.departmentBiological & Organometallic Catalysis Laboratories
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentChemical Science Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentKAUST Catalysis Center (KCC)
dc.contributor.departmentMolecular Signalling Group
dc.contributor.departmentOffice of the VP
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentPlant Science
dc.identifier.journalMolecules (Basel, Switzerland)
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Chemistry, Technische Universität München, Lichtenbergstr. 4, 85748 Garching, Germany
dc.contributor.institutionCollege of Science and Technology, Wenzhou-Kean University, 88 Daxue Road, Ouhai, Wenzhou 325060, Zhejiang, China
dc.contributor.institutionDepartment of Chemistry, Biology & Biotechnology, University of Perugia, Borgo XX giugno, 74, 06121 Perugia, Italy
kaust.personZarban, Randa Alhassan Yahya
kaust.personVogler, Malvina
kaust.personEppinger, Jorg
kaust.personAl-Babili, Salim
kaust.personGehring, Christoph A.
refterms.dateFOA2019-08-18T08:50:08Z


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by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).