Discovery of a Nitric Oxide-Responsive Protein in Arabidopsis thaliana.
AuthorsZarban, Randa Alhassan Yahya
Vogler, Malvina M.
Wong, Aloysius Tze
Gehring, Christoph A
KAUST DepartmentBiological & Organometallic Catalysis Laboratories
Biological and Environmental Sciences and Engineering (BESE) Division
Chemical Science Program
Desert Agriculture Initiative
KAUST Catalysis Center (KCC)
Molecular Signalling Group
Office of the VP
Physical Science and Engineering (PSE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/656458
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AbstractIn plants, much like in animals, nitric oxide (NO) has been established as an important gaseous signaling molecule. However, contrary to animal systems, NO-sensitive or NO-responsive proteins that bind NO in the form of a sensor or participating in redox reactions have remained elusive. Here, we applied a search term constructed based on conserved and functionally annotated amino acids at the centers of Heme Nitric Oxide/Oxygen (H-NOX) domains in annotated and experimentally-tested gas-binding proteins from lower and higher eukaryotes, in order to identify candidate NO-binding proteins in Arabidopsis thaliana. The selection of candidate NO-binding proteins identified from the motif search was supported by structural modeling. This approach identified AtLRB3 (At4g01160), a member of the Light Response Bric-a-Brac/Tramtrack/Broad Complex (BTB) family, as a candidate NO-binding protein. AtLRB3 was heterologously expressed and purified, and then tested for NO-response. Spectroscopic data confirmed that AtLRB3 contains a histidine-ligated heme cofactor and importantly, the addition of NO to AtLRB3 yielded absorption characteristics reminiscent of canonical H-NOX proteins. Furthermore, substitution of the heme iron-coordinating histidine at the H-NOX center with a leucine strongly impaired the NO-response. Our finding therefore established AtLRB3 as a NO-interacting protein and future characterizations will focus on resolving the nature of this response.
CitationZarban, R., Vogler, M., Wong, A., Eppinger, J., Al-Babili, S., & Gehring, C. (2019). Discovery of a Nitric Oxide-Responsive Protein in Arabidopsis thaliana. Molecules, 24(15), 2691. doi:10.3390/molecules24152691
SponsorsThe work was supported by King Abdullah University of Science and Technology and in particular, doctoral fellowships to R.Z. and M.V. A.W. is supported by the National Natural Science Foundation of China (Grant no. 31850410470) and the Zhejiang Provincial Natural Science Foundation of China (Grant no. LQ19C130001).
JournalMolecules (Basel, Switzerland)
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