Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts.
Type
ArticleAuthors
Darekar, Suhas DMushtaq, Muhammad

Gurrapu, Sreeharsha
Kovalevska, Larysa
Drummond, Catherine

Petruchek, Maria
Tirinato, Luca

Di Fabrizio, Enzo M.

Carbone, Ennio

Kashuba, Elena
KAUST Department
Material Science and Engineering ProgramPhysical Science and Engineering (PSE) Division
Date
2015-05-12Online Publication Date
2015-05-12Print Publication Date
2015-08-28Permanent link to this record
http://hdl.handle.net/10754/656440
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We have shown earlier that overexpression of the human mitochondrial ribosomal protein MRPS18-2 (S18-2) led to immortalization of primary rat embryonic fibroblasts. The derived cells expressed the embryonic stem cell markers, and cellular pathways that control cell proliferation, oxidative phosphorylation, cellular respiration, and other redox reactions were activated in the immortalized cells.Here we report that, upon overexpression of S18-2 protein, primary rat skin fibroblasts underwent cell transformation. Cells passed more than 300 population doublings, and two out of three tested clones gave rise to tumors in experimental animals. Transformed cells showed anchorage-independent growth and loss of contact inhibition; they expressed epithelial markers, such as E-cadherin and β-catenin. Transformed cells showed increased telomerase activity, disturbance of the cell cycle, and chromosomal instability. Taken together, our data suggest that S18-2 is a newly identified oncoprotein that may be involved in cancerogenesis.Citation
Darekar, S. D., Mushtaq, M., Gurrapu, S., Kovalevska, L., Drummond, C., Petruchek, M., … Kashuba, E. (2015). Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts. Oncotarget, 6(25). doi:10.18632/oncotarget.4123Publisher
Impact Journals, LLCJournal
OncotargetAdditional Links
http://www.oncotarget.com/fulltext/4123ae974a485f413a2113503eed53cd6c53
10.18632/oncotarget.4123
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