Key Role of Cytochrome C for Apoptosis Detection Using Raman Microimaging in an Animal Model of Brain Ischemia with Insulin Treatment.
Type
ArticleAuthors
Russo, VanessaCandeloro, Patrizio
Malara, Natalia
Perozziello, Gerardo
Iannone, Michelangelo
Scicchitano, Miriam
Mollace, Rocco
Musolino, Vincenzo
Gliozzi, Micaela
Carresi, Cristina
Morittu, Valeria M
Gratteri, Santo
Palma, Ernesto
Muscoli, Carolina
Di Fabrizio, Enzo M.

Mollace, Vincenzo
KAUST Department
Material Science and Engineering ProgramPhysical Science and Engineering (PSE) Division
Date
2019-07-12Permanent link to this record
http://hdl.handle.net/10754/656412
Metadata
Show full item recordAbstract
Brain ischemia represents a leading cause of death and disability in industrialized countries. To date, therapeutic intervention is largely unsatisfactory and novel strategies are required for getting better protection of neurons injured by cerebral blood flow restriction. Recent evidence suggests that brain insulin leads to protection of neuronal population undergoing apoptotic cell death via modulation of oxidative stress and mitochondrial cytochrome c (CytC), an effect to be better clarified. In this work, we investigate on the effect of insulin given intracerebroventricular (ICV) before inducing a transient global ischemia by bilateral occlusion of the common carotid arteries (BCCO) in Mongolian gerbils (MG). The transient (3 min) global ischemia in MG is observed to produce neurodegenerative effect mainly into CA3 hippocampal region, 72 h after cerebral blood restriction. Intracerebroventricular microinfusion of insulin significantly prevents the apoptosis of CA3 hippocampal neurons. Histological observation, after hematoxylin and eosin staining, puts in evidence the neuroprotective role of insulin, but Raman microimaging provides a clearer insight in the CytC mechanism underlying the apoptotic process. Above all, CytC has been revealed to be an outstanding, innate Raman marker for monitoring the cells status, thanks to its resonant scattering at 530 nm of incident wavelength and to its crucial role in the early stages of cells apoptosis. These data support the hypothesis of an insulin-dependent neuroprotection and antiapoptotic mechanism occurring in the brain of MG undergoing transient brain ischemia. The observed effects occurred without any peripheral change on serum glucose levels, suggesting an alternative mechanism of insulin-induced neuroprotection.Citation
Russo, V., Candeloro, P., Malara, N., Perozziello, G., Iannone, M., Scicchitano, M., … Mollace, V. (2019). Key Role of Cytochrome C for Apoptosis Detection Using Raman Microimaging in an Animal Model of Brain Ischemia with Insulin Treatment. Applied Spectroscopy, 000370281985867. doi:10.1177/0003702819858671Publisher
SAGE PublicationsJournal
Applied spectroscopyAdditional Links
http://journals.sagepub.com/doi/10.1177/0003702819858671ae974a485f413a2113503eed53cd6c53
10.1177/0003702819858671