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dc.contributor.authorNi, Ming
dc.contributor.authorZhuo, Shuangmu
dc.contributor.authorIliescu, Ciprian
dc.contributor.authorSo, Peter T C
dc.contributor.authorMehta, Jodhbir S
dc.contributor.authorYu, Hanry
dc.contributor.authorHauser, Charlotte
dc.date.accessioned2019-08-05T13:53:08Z
dc.date.available2019-08-05T13:53:08Z
dc.date.issued2019-06-04
dc.identifier.citationNi, M., Zhuo, S., Iliescu, C., So, P. T. C., Mehta, J. S., Yu, H., & Hauser, C. A. E. (2019). Self-assembling amyloid-like peptides as exogenous second harmonic probes for bioimaging applications. Journal of Biophotonics, e201900065. doi:10.1002/jbio.201900065
dc.identifier.doi10.1002/jbio.201900065
dc.identifier.urihttp://hdl.handle.net/10754/656355
dc.description.abstractAmyloid-like peptides are an ideal model for the mechanistic study of amyloidosis, which may lead to many human diseases, such as Alzheimer disease. This study reports a strong second harmonic generation (SHG) effect of amyloid-like peptides, having a signal equivalent to or even higher than those of endogenous collagen fibers. Several amyloid-like peptides (both synthetic and natural) were examined under SHG microscopy and shown they are SHG-active. These peptides can also be observed inside cells (in vitro). This interesting property can make these amyloid-like peptides second harmonic probes for bioimaging applications. Furthermore, SHG microscopy can provide a simple and label-free approach to detect amyloidosis. Lattice corneal dystrophy was chosen as a model disease of amyloidosis. Morphological difference between normal and diseased human corneal biopsy samples can be easily recognized, proving that SHG can be a useful tool for disease diagnosis.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201900065
dc.rightsArchived with thanks to Journal of biophotonics
dc.subjectbioimaging
dc.subjectnonlinear optical materials
dc.subjectsecond harmonic generation
dc.subjectself-assembly
dc.subjectultrashort peptides
dc.titleSelf-assembling amyloid-like peptides as exogenous second harmonic probes for bioimaging applications.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalJournal of biophotonics
dc.eprint.versionPost-print
dc.contributor.institutionInstitute of Bioengineering and Nanotechnology, Singapore.
dc.contributor.institutionKey Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, P. R. China.
dc.contributor.institutionBIGHEART, National University of Singapore, Singapore.
dc.contributor.institutionBiosystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, Singapore.
dc.contributor.institutionSingapore Eye Institute and Singapore National Eye Center, Singapore.
kaust.personHauser, Charlotte
refterms.dateFOA2019-11-18T07:54:44Z


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