Self-assembling amyloid-like peptides as exogenous second harmonic probes for bioimaging applications.
So, Peter T C
Mehta, Jodhbir S
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Permanent link to this recordhttp://hdl.handle.net/10754/656355
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AbstractAmyloid-like peptides are an ideal model for the mechanistic study of amyloidosis, which may lead to many human diseases, such as Alzheimer disease. This study reports a strong second harmonic generation (SHG) effect of amyloid-like peptides, having a signal equivalent to or even higher than those of endogenous collagen fibers. Several amyloid-like peptides (both synthetic and natural) were examined under SHG microscopy and shown they are SHG-active. These peptides can also be observed inside cells (in vitro). This interesting property can make these amyloid-like peptides second harmonic probes for bioimaging applications. Furthermore, SHG microscopy can provide a simple and label-free approach to detect amyloidosis. Lattice corneal dystrophy was chosen as a model disease of amyloidosis. Morphological difference between normal and diseased human corneal biopsy samples can be easily recognized, proving that SHG can be a useful tool for disease diagnosis.
CitationNi, M., Zhuo, S., Iliescu, C., So, P. T. C., Mehta, J. S., Yu, H., & Hauser, C. A. E. (2019). Self-assembling amyloid-like peptides as exogenous second harmonic probes for bioimaging applications. Journal of Biophotonics, e201900065. doi:10.1002/jbio.201900065
JournalJournal of biophotonics