AuthorsAcinas, Silvia G.
Cornejo-Castillo, Francisco M.
González, José M.
Arrieta, Jesús M.
Alam, Intikhab S.
Bajic, Vladimir B.
Sullivan, Matthew B.
Duarte, Carlos M.
Gasol, Josep M.
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Red Sea Research Center (RSRC)
Applied Mathematics and Computational Science Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
KAUST Grant NumberOSR #3362
Permanent link to this recordhttp://hdl.handle.net/10754/656339
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AbstractThe deep sea, the largest compartment of the ocean, is an essential component of the Earth system, but the functional exploration of its microbial communities lags far behind that of other marine realms. Here we analyze 58 bathypelagic microbial metagenomes from the Atlantic, Indian, and Pacific Oceans in an unprecedented sampling effort from the Malaspina Global Expedition, to resolve the metabolic architecture of the deep ocean microbiome. The Malaspina Deep-Sea Gene Collection, 71% of which consists of novel genes, reveals a strong dichotomy between the functional traits of free-living and particle-attached microorganisms, and shows relatively patchy composition challenging the paradigm of a uniform dark ocean ecosystem. Metagenome Assembled Genomes uncovered 11 potential new phyla, establishing references for deep ocean microbial taxa, and revealed mixotrophy to be a widespread trophic strategy in the deep ocean. These results expand our understanding of the functional diversity, metabolic versatility, and carbon cycling in the largest ecosystem on Earth.
CitationAcinas, S. G., Sánchez, P., Salazar, G., Cornejo-Castillo, F. M., Sebastián, M., Logares, R., … Gasol, J. M. (2019). Metabolic Architecture of the Deep Ocean Microbiome. doi:10.1101/635680
SponsorsThis work was funded by the Spanish Ministry of Economy and Competitiveness (MINECO) through the Consolider-Ingenio program (Malaspina 2010 Expedition, ref. CSD2008-00077). The sequencing of 58 bathypelagic metagenomes was done by the U.S. Department of Energy Joint Genome Institute, supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02 05CH11231 to SGA. Additional funding was provided by the project MAGGY (CTM2017-87736-R) to SGA from the Spanish Ministry of Economy and Competitiveness, Grup de Recerca 2017SGR/1568 from Generalitat de Catalunya, and King Abdullah University of Science and Technology (KAUST) under contract OSR #3362. HighPerformance computing analyses were run at the Marine Bioinformatics Service (MARBITS) of the Institut de Ciències del Mar (ICM-CSIC), Barcelona Supercomputing Center (Grant BCV2013-2-0001) and KAUST's Ibex HPC. We thank the R/V Hesperides crew, the chief scientists in Malaspina legs, and all project participants for their help in making this project possible. We thank Shook Studio for assistance with figure design and execution.
PublisherCold Spring Harbor Laboratory