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dc.contributor.authorZeaiter, Zahraa
dc.contributor.authorMarasco, Ramona
dc.contributor.authorBooth, Jenny Marie
dc.contributor.authorProsdocimi, Erica M.
dc.contributor.authorMapelli, Francesca
dc.contributor.authorCallegari, Matteo
dc.contributor.authorFusi, Marco
dc.contributor.authorMichoud, Gregoire
dc.contributor.authorMolinari, Francesco
dc.contributor.authorDaffonchio, Daniele
dc.contributor.authorBorin, Sara
dc.contributor.authorCrotti, Elena
dc.date.accessioned2019-08-04T12:25:16Z
dc.date.available2019-08-04T12:25:16Z
dc.date.issued2019-06-12
dc.identifier.citationZeaiter, Z., Marasco, R., Booth, J. M., Prosdocimi, E. M., Mapelli, F., Callegari, M., … Crotti, E. (2019). Phenomics and Genomics Reveal Adaptation of Virgibacillus dokdonensis Strain 21D to Its Origin of Isolation, the Seawater-Brine Interface of the Mediterranean Sea Deep Hypersaline Anoxic Basin Discovery. Frontiers in Microbiology, 10. doi:10.3389/fmicb.2019.01304
dc.identifier.doi10.3389/fmicb.2019.01304
dc.identifier.urihttp://hdl.handle.net/10754/656324
dc.description.abstractThe adaptation of sporeformers to extreme environmental conditions is frequently questioned due to their capacity to produce highly resistant endospores that are considered as resting contaminants, not representing populations adapted to the system. In this work, in order to gain a better understanding of bacterial adaptation to extreme habitats, we investigated the phenotypic and genomic characteristics of the halophile Virgibacillus sp. 21D isolated from the seawater-brine interface (SBI) of the MgCl2-saturated deep hypersaline anoxic basin Discovery located in the Eastern Mediterranean Sea. Vegetative cells of strain 21D showed the ability to grow in the presence of high concentrations of MgCl2, such as 14.28% corresponding to 1.5 M. Biolog phenotype MicroArray (PM) was adopted to investigate the strain phenotype, with reference to carbon energy utilization and osmotic tolerance. The strain was able to metabolize only 8.4% of 190 carbon sources provided in the PM1 and PM2 plates, mainly carbohydrates, in accordance with the low availability of nutrients in its habitat of origin. By using in silico DNA-DNA hybridization the analysis of strain 21D genome, assembled in one circular contig, revealed that the strain belongs to the species Virgibacillus dokdonensis. The genome presented compatible solute-based osmoadaptation traits, including genes encoding for osmotically activated glycine-betaine/carnitine/choline ABC transporters, as well as ectoine synthase enzymes. Osmoadaptation of the strain was then confirmed with phenotypic assays by using the osmolyte PM9 Biolog plate and growth experiments. Furthermore, the neutral isoelectric point of the reconstructed proteome suggested that the strain osmoadaptation was mainly mediated by compatible solutes. The presence of genes involved in iron acquisition and metabolism indicated that osmoadaptation was tailored to the iron-depleted saline waters of the Discovery SBI. Overall, both phenomics and genomics highlighted the potential capability of V. dokdonensis 21D vegetative cells to adapt to the environmental conditions in Discovery SBI.
dc.description.sponsorshipThe research leading to these results received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 311975 (MACUMBA) and from the EU Horizon 2020 Project contract number 634486 (INMARE). King Abdullah University of Science and Technology supported the study through the baseline research funds to DD.
dc.publisherFrontiers Media SA
dc.relation.urlhttps://www.frontiersin.org/article/10.3389/fmicb.2019.01304/full
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titlePhenomics and Genomics Reveal Adaptation of Virgibacillus dokdonensis Strain 21D to Its Origin of Isolation, the Seawater-Brine Interface of the Mediterranean Sea Deep Hypersaline Anoxic Basin Discovery
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalFrontiers in Microbiology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milan, Italy
kaust.personMarasco, Ramona
kaust.personBooth, Jenny Marie
kaust.personCallegari, Matteo
kaust.personFusi, Marco
kaust.personMichoud, Gregoire
kaust.personDaffonchio, Daniele
refterms.dateFOA2019-08-04T12:27:53Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.