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    iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions

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    Type
    Article
    Authors
    Ming, Tinghong
    Geng, Lingxin
    Feng, Ying
    Lu, Chenyang
    Zhou, Jun
    Li, Yanyan
    Zhang, Dijun
    He, Shan
    Li, Ye
    Cheong, Lingzhi
    Su, Xiurong
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2019-05-29
    Permanent link to this record
    http://hdl.handle.net/10754/656308
    
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    Abstract
    Staphylococcus aureus (S. aureus) is an extremely halotolerant pathogenic bacterium with high osmotic stress tolerance, and it is frequently encountered in aquatic production and preservation. However, the mechanism underlying the extremely high osmotic stress tolerance of S. aureus remains unclear. In this study, the isobaric tags for relative and absolute quantification (iTRAQ) method was used to identify the differentially expressed proteins (DEPs) under different sodium chloride (NaCl) concentrations. Compared with the control group (0% NaCl), the 10 and 20% NaCl groups had 484 DEPs and 750 DEPs, respectively. Compared with the 10% NaCl group, the 20% NaCl group had 361 DEPs. Among the DEPs, proteins involved in fatty acid synthesis, proline/glycine betaine biosynthesis and transportation, stress tolerance, cell wall biosynthesis and the TCA cycle were upregulated, whereas proteins associated with biofilm formation and pathogenic infections were downregulated. The results obtained in this study indicate that under extremely high osmotic stress, modification of the cell membrane structure, increased biosynthesis and transportation of osmotic protectants, and redistribution of energy metabolism contribute to the osmotic stress tolerance of S. aureus, and the infectious ability of the bacteria may be limited. The aim of this study was to provide new insight into how S. aureus tolerates the high-salt conditions involved in aquatic production and preservation.
    Citation
    Ming, T., Geng, L., Feng, Y., Lu, C., Zhou, J., Li, Y., … Su, X. (2019). iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions. Frontiers in Microbiology, 10. doi:10.3389/fmicb.2019.01082
    Sponsors
    We would like to thank Nature Research Editing Service for English language editing.
    Publisher
    Frontiers Media SA
    Journal
    Frontiers in Microbiology
    DOI
    10.3389/fmicb.2019.01082
    Additional Links
    https://www.frontiersin.org/article/10.3389/fmicb.2019.01082/full
    ae974a485f413a2113503eed53cd6c53
    10.3389/fmicb.2019.01082
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division

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