Water Disinfection Byproducts Increase Natural Transformation Rates of Environmental DNA in Acinetobacter baylyi ADP1
AuthorsMantilla Calderon, David
Plewa, Michael J.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering
Environmental Science and Engineering Program
Water Desalination & Reuse Center
Water Desalination and Reuse Research Center (WDRC)
KAUST Grant NumberBAS/1/1033-01-01
Online Publication Date2019-05-03
Print Publication Date2019-06-04
Permanent link to this recordhttp://hdl.handle.net/10754/655976
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AbstractThe process of natural transformation allows for the stable uptake, integration, and functional expression of extracellular DNA. This mechanism of horizontal gene transfer has been widely linked to the acquisition of antibiotic resistance and virulence factors. Here, we demonstrate that bromoacetic acid (BAA)—a regulated drinking water disinfection byproduct (DBP)—can stimulate natural transformation rates in the model organism Acinetobacter baylyi ADP1. We demonstrate that transformation stimulation in response to BAA is concentration-dependent and is linked to the ability of this compound to generate DNA damage via oxidative stress. In presence of BAA, transcription of recA was upregulated 20–40% compared to the nontreated controls, indicating that this component of the DNA damage response could be associated with the increase in transformation. Other genes associated with DNA translocation across the cytoplasmic membrane (i.e., pilX, comA) did not exhibit increased transcription in the presence of BAA, indicating that the enhancement of transformation is not associated with increased translocation rates of environmental DNA. Overall, these results lead us to speculate that elevated recA transcription levels could lead to increased integration rates of foreign DNA within the recipient cell during DNA repair. Lastly, we show that an artificial DBP cocktail simulating the environmental concentrations of five water DBP classes stimulates natural transformation by almost 2-fold. The results of this study suggest that mutagens like DBPs may play an important role in enhancing the fixation rates of extracellular DNA in the environmental metagenome.
CitationMantilla-Calderon, D., Plewa, M. J., Michoud, G., Fodelianakis, S., Daffonchio, D., & Hong, P.-Y. (2019). Water Disinfection Byproducts Increase Natural Transformation Rates of Environmental DNA in Acinetobacter baylyi ADP1. Environmental Science & Technology, 53(11), 6520–6528. doi:10.1021/acs.est.9b00692
SponsorsThis study is supported by KAUST baseline funding BAS/1/1033-01-01 awarded to P.-Y.H.
PublisherAmerican Chemical Society (ACS)