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    Water Disinfection Byproducts Increase Natural Transformation Rates of Environmental DNA in Acinetobacter baylyi ADP1

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    Type
    Article
    Authors
    Mantilla Calderon, David cc
    Plewa, Michael J.
    Michoud, Gregoire cc
    Fodelianakis, Stelios
    Daffonchio, Daniele cc
    Hong, Pei-Ying cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience
    Bioscience Program
    Environmental Science and Engineering
    Environmental Science and Engineering Program
    Water Desalination & Reuse Center
    Water Desalination and Reuse Research Center (WDRC)
    KAUST Grant Number
    BAS/1/1033-01-01
    Date
    2019-05-03
    Online Publication Date
    2019-05-03
    Print Publication Date
    2019-06-04
    Permanent link to this record
    http://hdl.handle.net/10754/655976
    
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    Abstract
    The process of natural transformation allows for the stable uptake, integration, and functional expression of extracellular DNA. This mechanism of horizontal gene transfer has been widely linked to the acquisition of antibiotic resistance and virulence factors. Here, we demonstrate that bromoacetic acid (BAA)—a regulated drinking water disinfection byproduct (DBP)—can stimulate natural transformation rates in the model organism Acinetobacter baylyi ADP1. We demonstrate that transformation stimulation in response to BAA is concentration-dependent and is linked to the ability of this compound to generate DNA damage via oxidative stress. In presence of BAA, transcription of recA was upregulated 20–40% compared to the nontreated controls, indicating that this component of the DNA damage response could be associated with the increase in transformation. Other genes associated with DNA translocation across the cytoplasmic membrane (i.e., pilX, comA) did not exhibit increased transcription in the presence of BAA, indicating that the enhancement of transformation is not associated with increased translocation rates of environmental DNA. Overall, these results lead us to speculate that elevated recA transcription levels could lead to increased integration rates of foreign DNA within the recipient cell during DNA repair. Lastly, we show that an artificial DBP cocktail simulating the environmental concentrations of five water DBP classes stimulates natural transformation by almost 2-fold. The results of this study suggest that mutagens like DBPs may play an important role in enhancing the fixation rates of extracellular DNA in the environmental metagenome.
    Citation
    Mantilla-Calderon, D., Plewa, M. J., Michoud, G., Fodelianakis, S., Daffonchio, D., & Hong, P.-Y. (2019). Water Disinfection Byproducts Increase Natural Transformation Rates of Environmental DNA in Acinetobacter baylyi ADP1. Environmental Science & Technology, 53(11), 6520–6528. doi:10.1021/acs.est.9b00692
    Sponsors
    This study is supported by KAUST baseline funding BAS/1/1033-01-01 awarded to P.-Y.H.
    Publisher
    American Chemical Society (ACS)
    Journal
    Environmental Science & Technology
    DOI
    10.1021/acs.est.9b00692
    Additional Links
    http://pubs.acs.org/doi/10.1021/acs.est.9b00692
    ae974a485f413a2113503eed53cd6c53
    10.1021/acs.est.9b00692
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)

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