Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation
KAUST DepartmentBioscience Core Lab
Permanent link to this recordhttp://hdl.handle.net/10754/653039
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AbstractPhosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.
CitationBarrios-Llerena ME, Le Bihan T (2019) Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation. Mass Spectrometry of Proteins: 35–42. Available: http://dx.doi.org/10.1007/978-1-4939-9232-4_4.
JournalMass Spectrometry of Proteins