AdvisorsArold, Stefan T.
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Embargo End Date2020-05-21
Permanent link to this recordhttp://hdl.handle.net/10754/652929
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Access RestrictionsAt the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2020-05-21.
AbstractCell-free transcription and translation systems promise to accelerate and simplify the engineering of synthetic proteins, biological circuits or metabolic pathways. Microfluidic droplet platforms can generate millions of reactions in parallel. This allows cell-free reactions to be miniaturized down to picoliter volumes. Nevertheless, the true potential of microfluidics have not been reached for cell-free bioengineering. Better approaches are needed for reaching sufficient in-drop expression levels while efficiently creating DNA diversity among droplets. This work develops a droplet microfluidic platform for single or multiple protein expression from a single DNA coated bead per droplet. This opens up the possibility to diversify a million droplets for synthetic biology applications.
CitationRestrepo, A. (2019). From DNA on beads to proteins in a million droplets. KAUST Research Repository. https://doi.org/10.25781/KAUST-3YIO1