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    From DNA on beads to proteins in a million droplets

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    AnaThesis_UPDATED.pdf
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    Description:
    Thesis
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    Type
    Thesis
    Authors
    Restrepo, Ana cc
    Advisors
    Arold, Stefan T. cc
    Committee members
    Hamdan, Samir cc
    Mahfouz, Magdy M. cc
    Program
    Bioscience
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2019-05
    Embargo End Date
    2020-05-21
    Permanent link to this record
    http://hdl.handle.net/10754/652929
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2020-05-21.
    Abstract
    Cell-free transcription and translation systems promise to accelerate and simplify the engineering of synthetic proteins, biological circuits or metabolic pathways. Microfluidic droplet platforms can generate millions of reactions in parallel. This allows cell-free reactions to be miniaturized down to picoliter volumes. Nevertheless, the true potential of microfluidics have not been reached for cell-free bioengineering. Better approaches are needed for reaching sufficient in-drop expression levels while efficiently creating DNA diversity among droplets. This work develops a droplet microfluidic platform for single or multiple protein expression from a single DNA coated bead per droplet. This opens up the possibility to diversify a million droplets for synthetic biology applications.
    Citation
    Restrepo, A. (2019). From DNA on beads to proteins in a million droplets. KAUST Research Repository. https://doi.org/10.25781/KAUST-3YIO1
    DOI
    10.25781/KAUST-3YIO1
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-3YIO1
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; MS Theses

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