Type
ThesisAuthors
Restrepo, Ana
Advisors
Arold, Stefan T.
Committee members
Hamdan, Samir
Mahfouz, Magdy M.

Program
BioscienceKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionDate
2019-05Embargo End Date
2020-05-21Permanent link to this record
http://hdl.handle.net/10754/652929
Metadata
Show full item recordAccess Restrictions
At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2020-05-21.Abstract
Cell-free transcription and translation systems promise to accelerate and simplify the engineering of synthetic proteins, biological circuits or metabolic pathways. Microfluidic droplet platforms can generate millions of reactions in parallel. This allows cell-free reactions to be miniaturized down to picoliter volumes. Nevertheless, the true potential of microfluidics have not been reached for cell-free bioengineering. Better approaches are needed for reaching sufficient in-drop expression levels while efficiently creating DNA diversity among droplets. This work develops a droplet microfluidic platform for single or multiple protein expression from a single DNA coated bead per droplet. This opens up the possibility to diversify a million droplets for synthetic biology applications.Citation
Restrepo, A. (2019). From DNA on beads to proteins in a million droplets. KAUST Research Repository. https://doi.org/10.25781/KAUST-3YIO1ae974a485f413a2113503eed53cd6c53
10.25781/KAUST-3YIO1