Show simple item record

dc.contributor.authorLaptenok, Sergey P
dc.contributor.authorRajamanickam, Vijayakumar Palanisamy
dc.contributor.authorGenchi, Luca
dc.contributor.authorMonfort, Tual
dc.contributor.authorLee, Yeonwoo
dc.contributor.authorPatel, Imran I
dc.contributor.authorBertoncini, Andrea
dc.contributor.authorLiberale, Carlo
dc.date.accessioned2019-05-15T06:54:45Z
dc.date.available2019-05-15T06:54:45Z
dc.date.issued2019-05-13
dc.identifier.citationLaptenok SP, Rajamanickam VP, Genchi L, Monfort T, Lee Y, et al. (2019) Fingerprint-to-CH stretch continuously tunable high spectral resolution Stimulated Raman Scattering microscope. Journal of Biophotonics: e201900028. Available: http://dx.doi.org/10.1002/jbio.201900028.
dc.identifier.issn1864-063X
dc.identifier.issn1864-0648
dc.identifier.doi10.1002/jbio.201900028
dc.identifier.urihttp://hdl.handle.net/10754/652883
dc.description.abstractStimulated Raman scattering (SRS) microscopy is a label-free method generating images based on chemical contrast within samples, and has already shown its great potential for high-sensitivity and fast imaging of biological specimens. The capability of SRS to collect molecular vibrational signatures in bio-samples, coupled with the availability of powerful statistical analysis methods, allows quantitative chemical imaging of live cells with sub-cellular resolution. This application has substantially driven the development of new SRS microscopy platforms. Indeed, in recent years, there has been a constant effort on devising configurations able to rapidly collect Raman spectra from samples over a wide vibrational spectral range, as needed for quantitative analysis by using chemometric methods. In this paper an SRS microscope which exploits spectral shaping by a narrowband and rapidly tunable Acousto Optical Tunable Filter (AOTF) is presented. This microscope enables spectral scanning from the Raman fingerprint region to the CH-stretch region without any modification of the optical setup. Moreover, it features also a high enough spectral resolution to allow resolving Raman peaks in the crowded fingerprint region. Finally, application of the developed SRS microscope to broadband hyperspectral imaging of biological samples over a large spectral range from 800 cm-1 till 3600 cm-1 , is demonstrated. This article is protected by copyright. All rights reserved.
dc.description.sponsorshipThis publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2016-CRG5-3017.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201900028
dc.rightsArchived with thanks to Journal of Biophotonics
dc.subjectlipid droplets
dc.subjectHyperspectral Imaging
dc.subjectStimulated Raman Scattering
dc.subjectCoherent Raman Imaging
dc.titleFingerprint-to-CH stretch continuously tunable high spectral resolution Stimulated Raman Scattering microscope
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalJournal of Biophotonics
dc.eprint.versionPost-print
kaust.personLaptenok, Sergey P
kaust.personRajamanickam, Vijayakumar Palanisamy
kaust.personGenchi, Luca
kaust.personMonfort, Tual
kaust.personLee, Yeonwoo
kaust.personPatel, Imran I
kaust.personBertoncini, Andrea
kaust.personLiberale, Carlo
kaust.grant.numberOSR-2016-CRG5-3017
refterms.dateFOA2019-05-20T06:16:10Z


Files in this item

Thumbnail
Name:
Laptenok_et_al_revised manuscript.pdf
Size:
2.496Mb
Format:
PDF
Description:
Accepted Manuscript
Thumbnail
Name:
Supplementary Information_Laptenok et al.pdf
Size:
6.247Mb
Format:
PDF
Description:
Supplemental files

This item appears in the following Collection(s)

Show simple item record