Fingerprint-to-CH stretch continuously tunable high spectral resolution Stimulated Raman Scattering microscope
AuthorsLaptenok, Sergey P
Rajamanickam, Vijayakumar Palanisamy
Patel, Imran I
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
KAUST Grant NumberOSR-2016-CRG5-3017
Permanent link to this recordhttp://hdl.handle.net/10754/652883
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AbstractStimulated Raman scattering (SRS) microscopy is a label-free method generating images based on chemical contrast within samples, and has already shown its great potential for high-sensitivity and fast imaging of biological specimens. The capability of SRS to collect molecular vibrational signatures in bio-samples, coupled with the availability of powerful statistical analysis methods, allows quantitative chemical imaging of live cells with sub-cellular resolution. This application has substantially driven the development of new SRS microscopy platforms. Indeed, in recent years, there has been a constant effort on devising configurations able to rapidly collect Raman spectra from samples over a wide vibrational spectral range, as needed for quantitative analysis by using chemometric methods. In this paper an SRS microscope which exploits spectral shaping by a narrowband and rapidly tunable Acousto Optical Tunable Filter (AOTF) is presented. This microscope enables spectral scanning from the Raman fingerprint region to the CH-stretch region without any modification of the optical setup. Moreover, it features also a high enough spectral resolution to allow resolving Raman peaks in the crowded fingerprint region. Finally, application of the developed SRS microscope to broadband hyperspectral imaging of biological samples over a large spectral range from 800 cm-1 till 3600 cm-1 , is demonstrated. This article is protected by copyright. All rights reserved.
CitationLaptenok SP, Rajamanickam VP, Genchi L, Monfort T, Lee Y, et al. (2019) Fingerprint-to-CH stretch continuously tunable high spectral resolution Stimulated Raman Scattering microscope. Journal of Biophotonics: e201900028. Available: http://dx.doi.org/10.1002/jbio.201900028.
SponsorsThis publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2016-CRG5-3017.
JournalJournal of Biophotonics