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dc.contributor.authorMi, Jianing
dc.contributor.authorJia, Kunpeng
dc.contributor.authorBalakrishna, Aparna
dc.contributor.authorFeng, Qitong
dc.contributor.authorAl-Babili, Salim
dc.date.accessioned2019-05-13T11:37:48Z
dc.date.available2019-05-13T11:37:48Z
dc.date.issued2019-05-10
dc.identifier.citationMi J, Jia K-P, Balakrishna A, Feng Q, Al-Babili S (2019) A Highly Sensitive SPE Derivatization–UHPLC–MS Approach for Quantitative Profiling of Carotenoid-Derived Dialdehydes from Vegetables. Journal of Agricultural and Food Chemistry. Available: http://dx.doi.org/10.1021/acs.jafc.9b01749.
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.doi10.1021/acs.jafc.9b01749
dc.identifier.urihttp://hdl.handle.net/10754/652857
dc.description.abstractOxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.
dc.description.sponsorshipWe thank Dr. Magnus Rueping, KAUST, for providing the labeled DIAL standard. This work was supported by base line funding and a Competitive Research Grant (CRG2017) given to Salim Al-Babili by the King Abdullah University of Science and Technology (KAUST), and the Bill and Melinda Gates Foundation (Grant number: OPP1194472).
dc.publisherAmerican Chemical Society (ACS)
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/acs.jafc.9b01749
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jafc.9b01749.
dc.subjectFood diapocarotenoids
dc.subjectDiapocarotenoids chemical derivatization
dc.subjectDiapocarotenoids SPE
dc.subjectDiapocarotenoids profiling
dc.subjectUHPLC-MS
dc.titleA Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Science
dc.identifier.journalJournal of Agricultural and Food Chemistry
dc.eprint.versionPost-print
kaust.personMi, Jianing
kaust.personJia, Kunpeng
kaust.personBalakrishna, Aparna
kaust.personFeng, Qitong
kaust.personAl-Babili, Salim
kaust.grant.numberCRG2017
refterms.dateFOA2020-05-05T00:00:00Z


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