A Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionDesert Agriculture Initiative
Plant Science
KAUST Grant Number
CRG2017Date
2019-05-10Permanent link to this record
http://hdl.handle.net/10754/652857
Metadata
Show full item recordAbstract
Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.Citation
Mi J, Jia K-P, Balakrishna A, Feng Q, Al-Babili S (2019) A Highly Sensitive SPE Derivatization–UHPLC–MS Approach for Quantitative Profiling of Carotenoid-Derived Dialdehydes from Vegetables. Journal of Agricultural and Food Chemistry. Available: http://dx.doi.org/10.1021/acs.jafc.9b01749.Sponsors
We thank Dr. Magnus Rueping, KAUST, for providing the labeled DIAL standard. This work was supported by base line funding and a Competitive Research Grant (CRG2017) given to Salim Al-Babili by the King Abdullah University of Science and Technology (KAUST), and the Bill and Melinda Gates Foundation (Grant number: OPP1194472).Publisher
American Chemical Society (ACS)Additional Links
https://pubs.acs.org/doi/10.1021/acs.jafc.9b01749ae974a485f413a2113503eed53cd6c53
10.1021/acs.jafc.9b01749