A Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
KAUST Grant NumberCRG2017
Permanent link to this recordhttp://hdl.handle.net/10754/652857
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AbstractOxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.
CitationMi J, Jia K-P, Balakrishna A, Feng Q, Al-Babili S (2019) A Highly Sensitive SPE Derivatization–UHPLC–MS Approach for Quantitative Profiling of Carotenoid-Derived Dialdehydes from Vegetables. Journal of Agricultural and Food Chemistry. Available: http://dx.doi.org/10.1021/acs.jafc.9b01749.
SponsorsWe thank Dr. Magnus Rueping, KAUST, for providing the labeled DIAL standard. This work was supported by base line funding and a Competitive Research Grant (CRG2017) given to Salim Al-Babili by the King Abdullah University of Science and Technology (KAUST), and the Bill and Melinda Gates Foundation (Grant number: OPP1194472).
PublisherAmerican Chemical Society (ACS)