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    A Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables

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    Type
    Article
    Authors
    Mi, Jianing cc
    Jia, Kunpeng cc
    Balakrishna, Aparna
    Feng, Qitong
    Al-Babili, Salim cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Desert Agriculture Initiative
    Plant Science
    KAUST Grant Number
    CRG2017
    Date
    2019-05-10
    Permanent link to this record
    http://hdl.handle.net/10754/652857
    
    Metadata
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    Abstract
    Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.
    Citation
    Mi J, Jia K-P, Balakrishna A, Feng Q, Al-Babili S (2019) A Highly Sensitive SPE Derivatization–UHPLC–MS Approach for Quantitative Profiling of Carotenoid-Derived Dialdehydes from Vegetables. Journal of Agricultural and Food Chemistry. Available: http://dx.doi.org/10.1021/acs.jafc.9b01749.
    Sponsors
    We thank Dr. Magnus Rueping, KAUST, for providing the labeled DIAL standard. This work was supported by base line funding and a Competitive Research Grant (CRG2017) given to Salim Al-Babili by the King Abdullah University of Science and Technology (KAUST), and the Bill and Melinda Gates Foundation (Grant number: OPP1194472).
    Publisher
    American Chemical Society (ACS)
    Journal
    Journal of Agricultural and Food Chemistry
    DOI
    10.1021/acs.jafc.9b01749
    Additional Links
    https://pubs.acs.org/doi/10.1021/acs.jafc.9b01749
    ae974a485f413a2113503eed53cd6c53
    10.1021/acs.jafc.9b01749
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative

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