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dc.contributor.authorJamil, Muhammad
dc.contributor.authorKountche, Boubacar Amadou
dc.contributor.authorHaider, Imran
dc.contributor.authorWang, Jian You
dc.contributor.authorAldossary, Faisal
dc.contributor.authorZarban, Randa Alhassan Yahya
dc.contributor.authorJia, Kunpeng
dc.contributor.authorYonli, Djibril
dc.contributor.authorShahul Hameed, Umar F.
dc.contributor.authorTakahashi, Ikuo
dc.contributor.authorOta, Tsuyoshi
dc.contributor.authorArold, Stefan T.
dc.contributor.authorAsami, Tadao
dc.contributor.authorAl-Babili, Salim
dc.date.accessioned2019-05-13T11:35:29Z
dc.date.available2019-05-13T11:35:29Z
dc.date.issued2019-04-02
dc.identifier.citationJamil M, Kountche BA, Haider I, Wang JY, Aldossary F, et al. (2019) Methylation at the C-3′ in D-Ring of Strigolactone Analogs Reduces Biological Activity in Root Parasitic Plants and Rice. Frontiers in Plant Science 10. Available: http://dx.doi.org/10.3389/fpls.2019.00353.
dc.identifier.issn1664-462X
dc.identifier.doi10.3389/fpls.2019.00353
dc.identifier.urihttp://hdl.handle.net/10754/652842
dc.description.abstractStrigolactones (SLs) regulate plant development and induce seed germination in obligate root parasitic weeds, e.g. Striga spp. Because organic synthesis of natural SLs is laborious, there is a large need for easy-to-synthesize and efficient analogs. Here, we investigated the effect of a structural modification of the D-ring, a conserved structural element in SLs. We synthesized and investigated the activity of two analogs, MP13 and MP26, which differ from previously published AR8 and AR36 only in the absence of methylation at C-3′. The de-methylated MP13 and MP26 were much more efficient in regulating plant development and inducing Striga seed germination, compared with AR8. Hydrolysis assays performed with purified Striga SL receptor and docking of AR8 and MP13 to the corresponding active site confirmed and explained the higher activity. Field trials performed in a naturally Striga-infested African farmer’s field unraveled MP13 as a promising candidate for combating Striga by inducing germination in host’s absence. Our findings demonstrate that methylation of the C-3′ in D-ring in SL analogs has a negative impact on their activity and identify MP13 and, particularly, MP26 as potent SL analogs with simple structures, which can be employed to control Striga, a major threat to global food security.
dc.description.sponsorshipWe are thankful to Dr. Binne Zwanenburg for providing GR24, Dr. Junko Kyozuka for providing seeds of the rice d mutants, and Dr. Abdel Gabar Babiker for providing S.hermonthica seeds. Funding. This study was supported by the Bill & Melinda Gates Foundation grant OPP1136424 and King Abdullah University of Science and Technology.
dc.publisherFrontiers Media SA
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fpls.2019.00353/full
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectShoot branching
dc.subjectStriga hermonthica
dc.subjectStrigolactone analogs
dc.subjectStrigolactones
dc.subjectSuicidal germination
dc.titleMethylation at the C-3′ in D-Ring of Strigolactone Analogs Reduces Biological Activity in Root Parasitic Plants and Rice
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.identifier.journalFrontiers in Plant Science
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Environment and Agricultural Research (INERA), Ouagadougou, , Burkina Faso
dc.contributor.institutionGraduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, , , Japan
kaust.personJamil, Muhammad
kaust.personKountche, Boubacar Amadou
kaust.personHaider, Imran
kaust.personWang, Jian You
kaust.personAldossary, Faisal
kaust.personZarban, Randa Alhassan Yahya
kaust.personJia, Kunpeng
kaust.personShahul Hameed, Umar F.
kaust.personArold, Stefan T.
kaust.personAl-Babili, Salim
refterms.dateFOA2019-05-14T06:21:58Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.