Methylation at the C-3′ in D-Ring of Strigolactone Analogs Reduces Biological Activity in Root Parasitic Plants and Rice
Type
ArticleAuthors
Jamil, Muhammad
Kountche, Boubacar Amadou

Haider, Imran

Wang, Jian You

Aldossary, Faisal
Zarban, Randa Alhassan Yahya

Jia, Kunpeng

Yonli, Djibril
Shahul Hameed, Umar F.
Takahashi, Ikuo
Ota, Tsuyoshi
Arold, Stefan T.

Asami, Tadao
Al-Babili, Salim

KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Center for Desert Agriculture
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division
Plant Science
Structural Biology and Engineering
Date
2019-04-02Permanent link to this record
http://hdl.handle.net/10754/652842
Metadata
Show full item recordAbstract
Strigolactones (SLs) regulate plant development and induce seed germination in obligate root parasitic weeds, e.g. Striga spp. Because organic synthesis of natural SLs is laborious, there is a large need for easy-to-synthesize and efficient analogs. Here, we investigated the effect of a structural modification of the D-ring, a conserved structural element in SLs. We synthesized and investigated the activity of two analogs, MP13 and MP26, which differ from previously published AR8 and AR36 only in the absence of methylation at C-3′. The de-methylated MP13 and MP26 were much more efficient in regulating plant development and inducing Striga seed germination, compared with AR8. Hydrolysis assays performed with purified Striga SL receptor and docking of AR8 and MP13 to the corresponding active site confirmed and explained the higher activity. Field trials performed in a naturally Striga-infested African farmer’s field unraveled MP13 as a promising candidate for combating Striga by inducing germination in host’s absence. Our findings demonstrate that methylation of the C-3′ in D-ring in SL analogs has a negative impact on their activity and identify MP13 and, particularly, MP26 as potent SL analogs with simple structures, which can be employed to control Striga, a major threat to global food security.Citation
Jamil M, Kountche BA, Haider I, Wang JY, Aldossary F, et al. (2019) Methylation at the C-3′ in D-Ring of Strigolactone Analogs Reduces Biological Activity in Root Parasitic Plants and Rice. Frontiers in Plant Science 10. Available: http://dx.doi.org/10.3389/fpls.2019.00353.Sponsors
We are thankful to Dr. Binne Zwanenburg for providing GR24, Dr. Junko Kyozuka for providing seeds of the rice d mutants, and Dr. Abdel Gabar Babiker for providing S.hermonthica seeds. Funding. This study was supported by the Bill & Melinda Gates Foundation grant OPP1136424 and King Abdullah University of Science and Technology.Publisher
Frontiers Media SAJournal
Frontiers in Plant ScienceAdditional Links
https://www.frontiersin.org/articles/10.3389/fpls.2019.00353/fullae974a485f413a2113503eed53cd6c53
10.3389/fpls.2019.00353
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