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CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

dc.contributor.authorButt, Haroon
dc.contributor.authorEid, Ayman
dc.contributor.authorMomin, Afaque Ahmad Imtiyaz
dc.contributor.authorBazin, Jeremie
dc.contributor.authorCrespi, Martin
dc.contributor.authorArold, Stefan T.
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2019-05-13T11:34:15Z
dc.date.available2019-05-13T11:34:15Z
dc.date.issued2019-04-30
dc.identifier.citationButt H, Eid A, Momin AA, Bazin J, Crespi M, et al. (2019) CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors. Genome Biology 20. Available: http://dx.doi.org/10.1186/s13059-019-1680-9.
dc.identifier.issn1474-760X
dc.identifier.doi10.1186/s13059-019-1680-9
dc.identifier.urihttp://hdl.handle.net/10754/652834
dc.description.abstractIncreasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B1 mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of resistance to splicing inhibitors. Studies of the structural basis of the splicing inhibitor binding to SGRs corroborate the resistance phenotype. This directed evolution platform can be used to interrogate and evolve the molecular functions of key biomolecules and to engineer crop traits for improved performance and adaptation under climate change conditions.
dc.description.sponsorshipAcknowledgements: We would like to thank members of the genome engineering and synthetic biology laboratory at KAUST for their critical discussion and technical help in this work. Funding: This work is funded by KAUST-baseline funding to Magdy Mahfouz and Stefan Arold.
dc.publisherSpringer Nature
dc.relation.urlhttps://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1680-9
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDirected evolution
dc.subjectHerbicide resistance
dc.subjectGenome Engineering
dc.subjectSpliceosome
dc.subjectCrispr/cas9
dc.subjectSf3b1
dc.subjectHerboxidiene
dc.subjectSpliceostatin A
dc.subjectPladienolide B
dc.subjectSplicing Modulators
dc.subjectSf3b Complex
dc.titleCRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Science and Engineering (CEMSE) Division
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.contributor.departmentStructural Biology and Engineering
dc.identifier.journalGenome Biology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCNRS, INRA, Institute of Plant Sciences Paris-Saclay IPS2, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cite, Universite Paris-Saclay, Orsay, France.
kaust.personButt, Haroon
kaust.personEid, Ayman
kaust.personMomin, Afaque Ahmad Imtiyaz
kaust.personArold, Stefan T.
kaust.personMahfouz, Magdy M.
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.c.4488176
refterms.dateFOA2019-05-14T06:46:53Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Dataset]</i> <br/> Butt, H., Eid, A., Afaque Momin, Bazin, J., Crespi, M., Arold, S., &amp; Magdy Mahfouz. (2019). <i>CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors</i>. Figshare. https://doi.org/10.6084/M9.FIGSHARE.C.4488176. DOI: <a href="https://doi.org/10.6084/m9.figshare.c.4488176" >10.6084/m9.figshare.c.4488176</a> Handle: <a href="http://hdl.handle.net/10754/664686" >10754/664686</a></a></li></ul>
dc.date.published-online2019-04-30
dc.date.published-print2019-12


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.