• Login
    View Item 
    •   Home
    • Research
    • Book Chapters
    • View Item
    •   Home
    • Research
    • Book Chapters
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    KAUSTRep_Diaz-Galicia_Review.pdf
    Size:
    1.124Mb
    Format:
    PDF
    Description:
    Accepted Manuscript
    Download
    Type
    Book Chapter
    Authors
    Díaz Galicia, M. Escarlet
    Aldehaiman, Abdullah cc
    Hong, Seungbeom
    Arold, Stefan T. cc
    Grunberg, Raik
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    KAUST Grant Number
    URF/1/2602-01
    FCC/1/1976-21
    Date
    2019-03-26
    Online Publication Date
    2019-03-26
    Print Publication Date
    2019
    Permanent link to this record
    http://hdl.handle.net/10754/631891
    
    Metadata
    Show full item record
    Abstract
    Protein tyrosine kinases (PTKs) are key signaling molecules and important drug targets. Although the efficient recombinant production of active PTKs is important for both pharmaceutical industry and academic research, most PTKs are still obtained from conventional, expensive and time-consuming insect-cell based expression. Host toxicity, kinase inactivity, insolubility and heterogeneity are among the reasons thought to preclude PTK expression in Escherichia coli. Herein we review these presumed roadblocks and their possible solutions for bacterial expression of PTKs, and give an overview on kinase activity assays. Finally, we report our experiences and observations with the kinases Src, Lyn and FAK as examples to illustrate implementation, effects and pitfalls of E. coli expression and in vitro assaying of PTKs.
    Citation
    Díaz Galicia ME, Aldehaiman A, Hong S, Arold ST, Grünberg R (2019) Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls. Methods in Enzymology. Available: http://dx.doi.org/10.1016/bs.mie.2019.02.027.
    Sponsors
    This research was supported by the King Abdullah University of Science and Technology (KAUST) through the baseline fund and the Award No. URF/1/2602-01 and FCC/1/1976-21 from the Office of Sponsored Research (OSR). Supplemental data, detailed methods and complete sequences are available on http://github.com/strubelab/kinaseexpress.
    Publisher
    Elsevier BV
    Journal
    Methods in Enzymology
    DOI
    10.1016/bs.mie.2019.02.027
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S0076687919300461
    ae974a485f413a2113503eed53cd6c53
    10.1016/bs.mie.2019.02.027
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC); Book Chapters; Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

    entitlement

     
    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.