Show simple item record

dc.contributor.authorBoshoff, Willem H P
dc.contributor.authorPrins, Renée
dc.contributor.authorde Klerk, Corneli
dc.contributor.authorKrattinger, Simon G.
dc.contributor.authorBender, Cornelia M
dc.contributor.authorMaree, Gerrie J
dc.contributor.authorRothmann, Lisa
dc.contributor.authorPretorius, Zacharias A
dc.date.accessioned2019-03-28T07:14:27Z
dc.date.available2019-03-28T07:14:27Z
dc.date.issued2018-11-29
dc.identifier.citationBoshoff WHP, Prins R, de Klerk C, Krattinger SG, Bender CM, et al. (2019) Point Inoculation Method for Measuring Adult Plant Response of Wheat to Stripe Rust Infection. Plant Disease: PDIS–08–18–1312. Available: http://dx.doi.org/10.1094/pdis-08-18-1312-re.
dc.identifier.issn0191-2917
dc.identifier.issn1943-7692
dc.identifier.doi10.1094/pdis-08-18-1312-re
dc.identifier.urihttp://hdl.handle.net/10754/631763
dc.description.abstractDepending on the pathogenicity of the stripe rust fungus Puccinia striiformis f. sp. tritici, the nature of resistance in the wheat host plant, and the environment, a broad range of disease phenotypes can be expressed. Therefore, the phenotyping of partial adult plant stripe rust resistance requires reliable and repeatable procedures, especially under controlled conditions. In this study, the development of a flag leaf point inoculation method, which resulted in a 100% initial infection rate, is reported. Flag leaf inoculations were achieved by placing 6-mm antibiotic test paper discs, dipped into a urediniospore and water suspension and covered with water-proof plastic tape, on the adaxial side of leaves. Results from independent trials allowed for the statistical comparison of stripe rust lesion expansion rate in wheat entries that differ in resistance. The technique is inexpensive, reliable, and applicable to routine screening for adult plant response type, quantitative comparison of stripe rust progress, environmental influences, and pathogenicity of different isolates.
dc.publisherScientific Societies
dc.relation.urlhttps://apsjournals.apsnet.org/doi/10.1094/PDIS-08-18-1312-RE
dc.rightsArchived with thanks to Plant Disease
dc.titlePoint inoculation method for measuring adult plant response of wheat to stripe rust infection
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.identifier.journalPlant Disease
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Plant Sciences, University of the Free State, Bloemfontein 9300, South Africa.
dc.contributor.institutionDepartment of Genetics, Stellenbosch University, 7602, Stellenbosch, South Africa
dc.contributor.institutionCenGen (Pty.) Ltd., Worcester 6850, South Africa.
kaust.personKrattinger, Simon G.
refterms.dateFOA2019-03-28T07:16:13Z
dc.date.published-online2018-11-29
dc.date.published-print2019-06


Files in this item

Thumbnail
Name:
Gh point inoculation article_SK.pdf
Size:
1022.Kb
Format:
PDF
Description:
Accepted Manuscript

This item appears in the following Collection(s)

Show simple item record