Point inoculation method for measuring adult plant response of wheat to stripe rust infection

Boshoff, Willem H P; Prins, Renée; de Klerk, Corneli; Krattinger, Simon G.; Bender, Cornelia M; Maree, Gerrie J; Rothmann, Lisa; Pretorius, Zacharias A

Point inoculation method for measuring adult plant response of wheat to stripe rust infection

Files

Gh point inoculation article_SK.pdf (1022.13 KB)

Type

Article

Authors

Boshoff, Willem H P
Prins, Renée
de Klerk, Corneli
Krattinger, Simon G.

Bender, Cornelia M
Maree, Gerrie J
Rothmann, Lisa
Pretorius, Zacharias A

KAUST Department

Biological and Environmental Sciences and Engineering (BESE) Division
Plant Science

Online Publication Date

2018-11-29

Print Publication Date

2019-06

Date

2018-11-29

Abstract

Depending on the pathogenicity of the stripe rust fungus Puccinia striiformis f. sp. tritici, the nature of resistance in the wheat host plant, and the environment, a broad range of disease phenotypes can be expressed. Therefore, the phenotyping of partial adult plant stripe rust resistance requires reliable and repeatable procedures, especially under controlled conditions. In this study, the development of a flag leaf point inoculation method, which resulted in a 100% initial infection rate, is reported. Flag leaf inoculations were achieved by placing 6-mm antibiotic test paper discs, dipped into a urediniospore and water suspension and covered with water-proof plastic tape, on the adaxial side of leaves. Results from independent trials allowed for the statistical comparison of stripe rust lesion expansion rate in wheat entries that differ in resistance. The technique is inexpensive, reliable, and applicable to routine screening for adult plant response type, quantitative comparison of stripe rust progress, environmental influences, and pathogenicity of different isolates.

Citation

Boshoff WHP, Prins R, de Klerk C, Krattinger SG, Bender CM, et al. (2019) Point Inoculation Method for Measuring Adult Plant Response of Wheat to Stripe Rust Infection. Plant Disease: PDIS–08–18–1312. Available: http://dx.doi.org/10.1094/pdis-08-18-1312-re.