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dc.contributor.authorLoo, Yihua
dc.contributor.authorChan, Yun S.
dc.contributor.authorSzczerbinska, Iwona
dc.contributor.authorTan, Bobby C. P.
dc.contributor.authorWan, Andrew C.A
dc.contributor.authorNg, Huck Hui
dc.contributor.authorHauser, Charlotte
dc.date.accessioned2019-03-11T07:16:50Z
dc.date.available2019-03-11T07:16:50Z
dc.date.issued2019-03
dc.identifier.citationLoo Y, Chan YS, Szczerbinska I, Tan BCP, Wan AC., et al. (2019) A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells. ACS Applied Bio Materials. Available: http://dx.doi.org/10.1021/acsabm.8b00686.
dc.identifier.issn2576-6422
dc.identifier.issn2576-6422
dc.identifier.doi10.1021/acsabm.8b00686
dc.identifier.urihttp://hdl.handle.net/10754/631541
dc.description.abstractClinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.
dc.description.sponsorshipThis work was supported by the Institute of Bioengineering and Nanotechnology, Genome Institute of Singapore, and Biomedical Research Council (Agency for Science, Technology and Research, Singapore), as well as by grant funding to Charlotte A. E. Hauser from the Agency for Science, Technology and Research (A*STAR) Joint Council Office (JCO) Grant 1231AFG023. Yihua Loo was also supported by the A*STAR BMRC YIG Grant 14/1/07/51/011.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/acsabm.8b00686
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Bio Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acsabm.8b00686.
dc.subjectXeno-free nanofibrous scaffolds
dc.subjectUltrashort peptides
dc.subjectSelf-assembled peptide hydrogels
dc.subjectHuman pluripotent stem cells
dc.subjectDirected 3D differentiation
dc.titleA Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.identifier.journalACS Applied Bio Materials
dc.eprint.versionPost-print
dc.contributor.institutionInstitute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #07-01, Singapore 138669.
dc.contributor.institutionGenome Institute of Singapore, 60 Biopolis Street, Genome, #02-01, Singapore 138672.
kaust.personHauser, Charlotte
refterms.dateFOA2020-03-01T00:00:00Z
dc.date.published-online2019-03
dc.date.published-print2019-04-15


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