A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells
Chan, Yun S.
Tan, Bobby C. P.
Wan, Andrew C.A
Ng, Huck Hui
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Biological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/631541
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AbstractClinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.
CitationLoo Y, Chan YS, Szczerbinska I, Tan BCP, Wan AC., et al. (2019) A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells. ACS Applied Bio Materials. Available: http://dx.doi.org/10.1021/acsabm.8b00686.
SponsorsThis work was supported by the Institute of Bioengineering and Nanotechnology, Genome Institute of Singapore, and Biomedical Research Council (Agency for Science, Technology and Research, Singapore), as well as by grant funding to Charlotte A. E. Hauser from the Agency for Science, Technology and Research (A*STAR) Joint Council Office (JCO) Grant 1231AFG023. Yihua Loo was also supported by the A*STAR BMRC YIG Grant 14/1/07/51/011.
PublisherAmerican Chemical Society (ACS)
JournalACS Applied Bio Materials