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dc.contributor.authorValpapuram, Immanuel
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorColuccio, Maria
dc.contributor.authorParrotta, Elvira
dc.contributor.authorGiugni, Andrea
dc.contributor.authorDas, Gobind
dc.contributor.authorCuda, Gianni
dc.contributor.authorDi Fabrizio, Enzo M.
dc.contributor.authorPerozziello, Gerardo
dc.date.accessioned2019-03-11T07:15:23Z
dc.date.available2019-03-11T07:15:23Z
dc.date.issued2019-03-03
dc.identifier.citationValpapuram I, Candeloro P, Coluccio M, Parrotta E, Giugni A, et al. (2019) Waveguiding and SERS Simplified Raman Spectroscopy on Biological Samples. Biosensors 9: 37. Available: http://dx.doi.org/10.3390/bios9010037.
dc.identifier.issn2079-6374
dc.identifier.doi10.3390/bios9010037
dc.identifier.urihttp://hdl.handle.net/10754/631532
dc.description.abstractBiomarkers detection at an ultra-low concentration in biofluids (blood, serum, saliva, etc.) is a key point for the early diagnosis success and the development of personalized therapies. However, it remains a challenge due to limiting factors like (i) the complexity of analyzed media, and (ii) the aspecificity detection and the poor sensitivity of the conventional methods. In addition, several applications require the integration of the primary sensors with other devices (microfluidic devices, capillaries, flasks, vials, etc.) where transducing the signal might be difficult, reducing performances and applicability. In the present work, we demonstrate a new class of optical biosensor we have developed integrating an optical waveguide (OWG) with specific plasmonic surfaces. Exploiting the plasmonic resonance, the devices give consistent results in surface enhanced Raman spectroscopy (SERS) for continuous and label-free detection of biological compounds. The OWG allows driving optical signals in the proximity of SERS surfaces (detection area) overcoming spatial constraints, in order to reach places previously optically inaccessible. A rutile prism couples the remote laser source to the OWG, while a Raman spectrometer collects the SERS far field scattering. The present biosensors were implemented by a simple fabrication process, which includes photolithography and nanofabrication. By using such devices, it was possible to detect cell metabolites like Phenylalanine (Phe), Adenosine 5-triphosphate sodium hydrate (ATP), Sodium Lactate, Human Interleukin 6 (IL6), and relate them to possible metabolic pathway variation.
dc.description.sponsorshipThis work was partially supported by the project for Young researchers financed from the Ministry of Health “High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro and the CRG5 project Grant #URF/1/2998-01 funded by KAUST OSR research Service.
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/2079-6374/9/1/37
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectprism coupling
dc.subjectoptical waveguide
dc.subjectoptical biosensors
dc.subjectRaman micro-spectroscopy
dc.titleWaveguiding and SERS Simplified Raman Spectroscopy on Biological Samples
dc.typeArticle
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Characterization
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalBiosensors
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, Italy
kaust.personGiugni, Andrea
kaust.personDas, Gobind
kaust.personDi Fabrizio, Enzo M.
kaust.grant.numberURF/1/2998-01
refterms.dateFOA2019-03-12T12:20:34Z


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).