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dc.contributor.authorArtiles, Karen L
dc.contributor.authorFire, Andrew Z
dc.contributor.authorFrøkjær-Jensen, Christian
dc.date.accessioned2019-03-04T08:23:23Z
dc.date.available2019-03-04T08:23:23Z
dc.date.issued2019-02-23
dc.identifier.citationArtiles KL, Fire AZ, Frøkjær-Jensen C (2019) Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans. Developmental Cell. Available: http://dx.doi.org/10.1016/j.devcel.2019.01.020.
dc.identifier.issn1534-5807
dc.identifier.doi10.1016/j.devcel.2019.01.020
dc.identifier.urihttp://hdl.handle.net/10754/631308
dc.description.abstractThe recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.
dc.description.sponsorshipWe thank Henrik Bringmann for communications on the nature of non-canonical inheritance; Elif Sarinay Cenik and Sedona Murphy for observations on the gpr-1(OE) strains and their genetic interactions; Josh Arribere for the gfp-tagged unc-54 allele; Lamia Wahba for pointing out biological aspects of non-canonical inheritance; Loren Hansen for guidance in SNP calling; Tim Schedl, Michael Ailion, Maria Sallee, and Anne Villeneuve for helpful discussions; Tomoko Tabuchi and Susan Strome for communicating results prior to publication; and Elif Sarinay Cenik, Nimit Jain, Massa Shoura, and Ryan Bell for their critical reading of the manuscript. This work was supported by grant NIGMS-R01-GM37706/GM130366 (to A.Z.F.). Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440).
dc.publisherElsevier BV
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S1534580719300486
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Developmental Cell. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Developmental Cell, February 21, 2019. DOI: 10.1016/j.devcel.2019.01.020. © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectC. elegans
dc.subjectnon-Mendelian
dc.subjectinheritance
dc.subjectmosaic
dc.subjectmitosis
dc.subjectgenetic engineering
dc.subjectsynthetic biology
dc.titleAssessment and Maintenance of Unigametic Germline Inheritance for C. elegans
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentKAUST Environmental Epigenetics Research Program (KEEP)
dc.identifier.journalDevelopmental Cell
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
kaust.personFroekjaer Jensen, Christian
dc.date.published-online2019-02-23
dc.date.published-print2019-03


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