Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
KAUST Environmental Epigenetics Research Program (KEEP)
Online Publication Date2019-02-23
Print Publication Date2019-03
Permanent link to this recordhttp://hdl.handle.net/10754/631308
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AbstractThe recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.
CitationArtiles KL, Fire AZ, Frøkjær-Jensen C (2019) Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans. Developmental Cell. Available: http://dx.doi.org/10.1016/j.devcel.2019.01.020.
SponsorsWe thank Henrik Bringmann for communications on the nature of non-canonical inheritance; Elif Sarinay Cenik and Sedona Murphy for observations on the gpr-1(OE) strains and their genetic interactions; Josh Arribere for the gfp-tagged unc-54 allele; Lamia Wahba for pointing out biological aspects of non-canonical inheritance; Loren Hansen for guidance in SNP calling; Tim Schedl, Michael Ailion, Maria Sallee, and Anne Villeneuve for helpful discussions; Tomoko Tabuchi and Susan Strome for communicating results prior to publication; and Elif Sarinay Cenik, Nimit Jain, Massa Shoura, and Ryan Bell for their critical reading of the manuscript. This work was supported by grant NIGMS-R01-GM37706/GM130366 (to A.Z.F.). Some strains were provided by the CGC, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440).