Show simple item record

dc.contributor.authorCarvalho, Susana
dc.contributor.authorAylagas, Eva
dc.contributor.authorVillalobos, Rodrigo
dc.contributor.authorKattan, Yasser
dc.contributor.authorBerumen, Michael L.
dc.contributor.authorPearman, John K.
dc.date.accessioned2019-02-20T07:20:11Z
dc.date.available2019-02-20T07:20:11Z
dc.date.issued2019-02-13
dc.identifier.citationCarvalho S, Aylagas E, Villalobos R, Kattan Y, Berumen M, et al. (2019) Beyond the visual: using metabarcoding to characterize the hidden reef cryptobiome. Proceedings of the Royal Society B: Biological Sciences 286: 20182697. Available: http://dx.doi.org/10.1098/rspb.2018.2697.
dc.identifier.issn0962-8452
dc.identifier.issn1471-2954
dc.identifier.doi10.1098/rspb.2018.2697
dc.identifier.urihttp://hdl.handle.net/10754/631099
dc.description.abstractIn an era of coral reef degradation, our knowledge of ecological patterns in reefs is biased towards large conspicuous organisms. The majority of biodiversity, however, inhabits small cryptic spaces within the framework of the reef. To assess this biodiverse community, which we term the ‘reef cryptobiome’, we deployed 87 autonomous reef monitoring structures (ARMS), on 22 reefs across 16 degrees latitude of the Red Sea. Combining ARMS with metabarcoding of the mitochondrial cytochrome oxidase I gene, we reveal a rich community, including the identification of 14 metazoan phyla within 10 416 operational taxonomic units (OTUs). While mobile and sessile subsets were similarly structured along the basin, the main environmental driver was different (particulate organic matter and sea surface temperature, respectively). Distribution patterns of OTUs showed that only 1.5% were present in all reefs, while over half were present in a single reef. On both local and regional scales, the majority of OTUs were rare. The high heterogeneity in community patterns of the reef cryptobiome has implications for reef conservation. Understanding the biodiversity patterns of this critical component of reef functioning will enable a sound knowledge of how coral reefs will respond to future anthropogenic impacts.
dc.description.sponsorshipFunding: The research reported in this publication was supported by funding from a collaboration between KAUST and Saudi Aramco within the framework of the Saudi Aramco–KAUST Center for Marine Environmental Observations. Acknowledgements: We are grateful to the personnel from the King Abdullah University of Science and Technology (KAUST) Coastal and Marine Resources Core Lab for logistical support as well as to the crew of RV Thuwal. The authors would also like to thank Katherine Rowe, Joanne Ellis, Amr Gusti, Giuseppe Merlino, Aislinn Dunne, Florian Roth, Joao Curdia, Zahra Alsaffar, Alejandro Restrepo, Perdana Prihartato, Holger Anlauf, and members of the Red Sea Research Center summer student program 2017 for their help with the processing of the samples. We would also like to thank the editor and two reviewers who greatly contributed to improving an earlier version of this manuscript.
dc.publisherThe Royal Society
dc.relation.urlhttps://royalsocietypublishing.org/doi/10.1098/rspb.2018.2697
dc.rightsPublished by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectcoral reefs
dc.subjectautonomous reef monitoring structures
dc.subjectreef cryptobiome
dc.subjecteDNA biodiversity
dc.subjectcytochrome oxidase
dc.titleBeyond the visual: using metabarcoding to characterize the hidden reef cryptobiome
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.identifier.journalProceedings of the Royal Society B: Biological Sciences
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionEnvironmental Protection Department, Saudi Aramco, Dhahran 31311, Saudi Arabia
kaust.personCarvalho, Susana
kaust.personAylagas, Eva
kaust.personVillalobos, Rodrigo
kaust.personBerumen, Michael L.
kaust.personPearman, John K.
dc.relation.issupplementedbybioproject:PRJNA485689
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.c.4397870.v1
refterms.dateFOA2019-02-20T08:03:28Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Bioproject]</i> <br/> Title: Cryptobiome COI raw reads. Publication Date: 2018-08-13. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA485689" >PRJNA485689</a> Handle: <a href="http://hdl.handle.net/10754/666529" >10754/666529</a></a></li><li><i>[Dataset]</i> <br/> Carvalho, S., Aylagas, E., Villalobos, R., Kattan, Y., Berumen, M., &amp; Pearman, J. K. (2019). <i>Supplementary material from "Beyond the visual: using metabarcoding to characterize the hidden reef cryptobiome"</i>. Figshare. https://doi.org/10.6084/M9.FIGSHARE.C.4397870.V1. DOI: <a href="https://doi.org/10.6084/m9.figshare.c.4397870.v1" >10.6084/m9.figshare.c.4397870.v1</a> Handle: <a href="http://hdl.handle.net/10754/664477" >10754/664477</a></a></li></ul>
dc.date.published-online2019-02-13
dc.date.published-print2019-02-13


Files in this item

Thumbnail
Name:
rspb.2018.2697.pdf
Size:
1.801Mb
Format:
PDF
Description:
Published version
Thumbnail
Name:
rspb20182697supp1.jpeg
Size:
28.74Kb
Format:
JPEG image
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp2.eps
Size:
21.12Kb
Format:
Postscript
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp3.eps
Size:
76.65Kb
Format:
Postscript
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp4.eps
Size:
18.14Kb
Format:
Postscript
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp5.eps
Size:
12.28Kb
Format:
Postscript
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp6.xlsx
Size:
16.56Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp7.xlsx
Size:
17.08Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp8.xlsx
Size:
14.84Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Thumbnail
Name:
rspb20182697supp9.docx
Size:
19.54Kb
Format:
Microsoft Word 2007
Description:
Supplemental files

This item appears in the following Collection(s)

Show simple item record

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.