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    Thin Peptide Hydrogel Membranes Suitable as Scaffolds for Engineering Layered Biostructures

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    Type
    Article
    Authors
    Seow, Wei Yang
    Kandasamy, Karthikeyan
    Purnamawati, Kristy
    Sun, William
    Hauser, Charlotte cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Laboratory for Nanomedicine, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
    Date
    2019-02-02
    Online Publication Date
    2019-02-02
    Print Publication Date
    2019-04
    Permanent link to this record
    http://hdl.handle.net/10754/631044
    
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    Abstract
    A short tetramer peptide, Ac-IVKC, spontaneously formed a hydrogel in water. Disulfide bonds were introduced via hydrogen peroxide (H2O2)-assisted oxidation, resulting in (Ac-IVKC)2 dimers. The extent of disulfide bond formation and gel stiffness increased with the amount of H2O2 used and 100% dimerization was achieved with 0.2% H2O2. The resultant gel achieved an elastic modulus of ∼0.9 MPa, which to our knowledge, has not been reported for peptide-based hydrogels. The enhanced mechanical property enabled the fabrication of thin and transparent membranes. The hydrogel could also be handled with forceps at mm thickness, greatly increasing its ease of physical manipulation. Excess H2O2 was removed and the membrane was then infused with cell culture media. Various cells, including primary human corneal stromal and epithelial cells, were seeded onto the hydrogel membrane and demonstrated to remain viable. Depending on the intended application, specific cell combination or membrane stacking order could be used to engineer layered biostructures. STATEMENT OF SIGNIFICANCE: A short tetramer peptide - Ac-IVKC - spontaneously formed a hydrogel in water and disulfide bonds were introduced via hydrogen peroxide (H2O2)-assisted oxidation. The extent of disulfide-bond formation and gel stiffness were modulated by the amount of H2O2. At maximum disulfide-bond formation, the hydrogel achieved an elastic modulus of ∼0.9 MPa, which to our knowledge, has not been reported for peptide-based hydrogels. The enhanced mechanical property enabled the fabrication of thin transparent membranes that can be physically manipulated at mm thickness. The gels also supported 3D cell growth, including primary human corneal stromal and epithelial cells. Depending on the intended application, specific combination of cells or individual membrane stacking order could be used to engineer layered biostructures.
    Citation
    Seow WY, Kandasamy K, Purnamawati K, Sun W, Hauser CAE (2019) Thin Peptide Hydrogel Membranes Suitable as Scaffolds for Engineering Layered Biostructures. Acta Biomaterialia. Available: http://dx.doi.org/10.1016/j.actbio.2019.02.001.
    Sponsors
    We thank the A*STAR Microscopy Platform for assistance in sample processing and ultrastructural analysis by TEM. This work was supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore).
    Publisher
    Elsevier BV
    Journal
    Acta Biomaterialia
    DOI
    10.1016/j.actbio.2019.02.001
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S1742706119301059
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.actbio.2019.02.001
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

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