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dc.contributor.authorLaMonte, Gregory M.
dc.contributor.authorOrjuela-Sanchez, Pamela
dc.contributor.authorCalla, Jaeson
dc.contributor.authorWang, Lawrence T.
dc.contributor.authorLi, Shangzhong
dc.contributor.authorSwann, Justine
dc.contributor.authorCowell, Annie N.
dc.contributor.authorZou, Bing Yu
dc.contributor.authorAbdel-Haleem, Alyaa M.
dc.contributor.authorVilla Galarce, Zaira Hellen
dc.contributor.authorMoreno, Marta
dc.contributor.authorTong Rios, Carlos
dc.contributor.authorVinetz, Joseph M.
dc.contributor.authorLewis, Nathan
dc.contributor.authorWinzeler, Elizabeth A.
dc.date.accessioned2019-02-14T08:19:51Z
dc.date.available2019-02-14T08:19:51Z
dc.date.issued2019-01-30
dc.identifier.citationLaMonte GM, Orjuela-Sanchez P, Calla J, Wang LT, Li S, et al. (2019) Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection. Nature Communications 10. Available: http://dx.doi.org/10.1038/s41467-019-08349-0.
dc.identifier.issn2041-1723
dc.identifier.doi10.1038/s41467-019-08349-0
dc.identifier.urihttp://hdl.handle.net/10754/631042
dc.description.abstractThe exoerythrocytic stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium exoerythrocytic hepatic-stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection.
dc.description.sponsorshipWe thank the members of the Winzeler and Lewis labs for advice and critical reading of the manuscript. In addition, we thank Medicines for Malaria Venture for all of their support of the insectary in Peru. We would also like to thank the UCSD Institute for Genomic Medicine Sequencing Core Facility and the UCSD Human Embryonic Stem Cell Flow Cytometry Core Facility for their technical support. G.L. is supported by an A.P. Giannini Post-Doctoral Fellowship. E.A.W. is supported by grants from the NIH (5R01AI090141 and R01AI103058). N.E.L. and S.L. received funding from the NIGMS (R35 GM119850) and the Novo Nordisk Foundation through the Center for Biosustainability at the Technical University of Denmark (NNF10CC1016517). The P. vivax work was supported by grants to J.M.V. from the NIH (D43TW007120 and U19AI089681). A.N.C. received support from a NIH T32 AI 007036 training grant.
dc.publisherSpringer Nature
dc.relation.urlhttps://www.nature.com/articles/s41467-019-08349-0
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleDual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalNature Communications
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, CA, 92093, USA.
dc.contributor.institutionDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, USA
dc.contributor.institutionNovo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, La Jolla, CA, 92093, USA
dc.contributor.institutionDivision of Infectious Diseases, Department of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
dc.contributor.institutionLaboratorio ICEMR-Amazonia, Laboratorio de Investigación y Desarrollo, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Lima, Peru.
kaust.personAbdel-Haleem, Alyaa M.
refterms.dateFOA2019-02-17T06:55:25Z
dc.date.published-online2019-01-30
dc.date.published-print2019-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.