Show simple item record

dc.contributor.authorLópez-Sandoval, Daffne C.
dc.contributor.authorDelgado-Huertas, Antonio
dc.contributor.authorCarrillo-de-Albornoz, Paloma
dc.contributor.authorDuarte, Carlos M.
dc.contributor.authorAgusti, Susana
dc.date.accessioned2019-01-22T12:10:53Z
dc.date.available2019-01-22T12:10:53Z
dc.date.issued2019-01-14
dc.identifier.citationLópez-Sandoval DC, Delgado-Huertas A, Carrillo-de-Albornoz P, Duarte CM, Agustí S (2019) Use of cavity ring-down spectrometry to quantify 13 C-primary productivity in oligotrophic waters. Limnology and Oceanography: Methods. Available: http://dx.doi.org/10.1002/lom3.10305.
dc.identifier.issn1541-5856
dc.identifier.doi10.1002/lom3.10305
dc.identifier.urihttp://hdl.handle.net/10754/630926
dc.description.abstractCavity ring-down spectroscopy (CRDS) is a highly sensitive laser technique that allows the analysis of isotopic signals and absolute concentration of individual molecular species in small-volume samples. Here, we describe a protocol to quantify photosynthetic 13C-uptake rates of marine phytoplankton by using the CRDS technique (13C-CRDS-PP). We validated our method by comparing the 13C-PP rates measured between CRDS and isotope ratio mass spectrometry (IRMS) in samples with different carbon content (30–160 μgC). The comparison revealed that 13C-CRDS-PP rates were highly correlated with those obtained by IRMS (Spearman correlation coefficient, ρ = 0.95, p < 0.0001, n = 15), with a mean difference between the two estimates of ± 0.08 mgC m−3 h−1. Moreover, the slope of the relationship between CRDS and IRMS results was not significantly different from 1 (F = 0.03, p = 0.86), and the intercept did not differ from 0 (F = 1.4, p = 0.24), indicating that there was no bias in the CRDS relative to the IRMS-based measurements. A separate analysis also showed that despite the difference in volume and carbon content between samples (40 ± 10 μgC and 160 ± 40 μgC, respectively), the 13C-CRDS-PP technique provides similar results (Mann–Whitney test, U = 30.5, p = 0.90, n = 8). In addition, 13C-CRDS-PP rates measured along the Red Sea (∼ 176 mgC m−2 d−1) agreed with 14C-based PP rates previously reported for similar locations. Thus, this study evidenced that the 13C-CRDS-PP method is sensitive enough to quantify carbon fixation rates in oligotrophic regions.
dc.description.sponsorshipThe research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST), under award number BAS/1/1071-01-10 assigned to CMD, BAS/1/1072-01-01 assigned to SA, and FCC/1/1973-21-01 assigned to the Red Sea Research Center. We also thank to the anonymous referees for their helpful and valuable comments and suggestions to improve our manuscript.
dc.publisherWiley
dc.relation.urlhttps://aslopubs.onlinelibrary.wiley.com/doi/full/10.1002/lom3.10305
dc.rightsThis is an open access article under the terms of the Creative CommonsAttribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleUse of cavity ring-down spectrometry to quantify 13 C-primary productivity in oligotrophic waters
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalLimnology and Oceanography: Methods
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstituto Andaluz de Ciencias de la Tierra, CSIC-UGR, Armilla, Spain.
kaust.personLopez Sandoval, Daffne
kaust.personCarrillo-de-Albornoz, Paloma
kaust.personDuarte, Carlos M.
kaust.personAgusti, Susana
kaust.grant.numberBAS/1/1071-01-10
kaust.grant.numberBAS/1/1072-01-01
kaust.grant.numberFCC/1/1973-21-01
refterms.dateFOA2019-01-24T10:23:11Z
dc.date.published-online2019-01-14
dc.date.published-print2019-02


Files in this item

Thumbnail
Name:
L-pez-Sandoval_et_al-2019-Limnology_and_Oceanography__Methods.pdf
Size:
667.5Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

This is an open access article under the terms of the Creative CommonsAttribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creative CommonsAttribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.