Role of MPK4 in pathogen-associated molecular pattern-triggered alternative splicing in Arabidopsis
Type
ArticleAuthors
Bazin, JeremieMariappan, Kiruthiga

Jiang, Yunhe

Blein, Thomas

Volz, Ronny

Crespi, Martin

Hirt, Heribert

KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionCenter for Desert Agriculture
DARWIN21, Biological and Environmental Sciences and Engineering Division, King AbdullahUniversity of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Hirt Lab
Plant Science
Red Sea Research Center (RSRC)
KAUST Grant Number
BAS/1/1062-01-01CRG4
OCRF-2014-CRG
Date
2020-04-17Submitted Date
2019-11-20Permanent link to this record
http://hdl.handle.net/10754/630774
Metadata
Show full item recordAbstract
Alternative splicing (AS) of pre-mRNAs in plants is an important mechanism of gene regulation in environmental stress tolerance but plant signals involved are essentially unknown. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) is mediated by mitogen-activated protein kinases and the majority of PTI defense genes are regulated by MPK3, MPK4 and MPK6. These responses have been mainly analyzed at the transcriptional level, however many splicing factors are direct targets of MAPKs. Here, we studied alternative splicing induced by the PAMP flagellin in Arabidopsis. We identified 506 PAMP-induced differentially alternatively spliced (DAS) genes. Importantly, of the 506 PAMP-induced DAS genes, only 89 overlap with the set of 1950 PAMP-induced differentially expressed genes (DEG), indicating that transcriptome analysis does not identify most DAS events. Global DAS analysis of mpk3, mpk4, and mpk6 mutants in the absence of PAMP treatment showed no major splicing changes. However, in contrast to MPK3 and MPK6, MPK4 was found to be a key regulator of PAMP-induced DAS events as the AS of a number of splicing factors and immunity-related protein kinases is affected, such as the calcium-dependent protein kinase CPK28, the cysteine-rich receptor like kinases CRK13 and CRK29 or the FLS2 co-receptor SERK4/BKK1. Although MPK4 is guarded by SUMM2 and consequently, the mpk4 dwarf and DEG phenotypes are suppressed in mpk4 summ2 mutants, MPK4-dependent DAS is not suppressed by SUMM2, supporting the notion that PAMP-triggered MPK4 activation mediates regulation of alternative splicing.Citation
Bazin, J., Mariappan, K., Jiang, Y., Blein, T., Voelz, R., Crespi, M., & Hirt, H. (2020). Role of MPK4 in pathogen-associated molecular pattern-triggered alternative splicing in Arabidopsis. PLOS Pathogens, 16(4), e1008401. doi:10.1371/journal.ppat.1008401Sponsors
This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) to Prof. Heribert Hirt No. BAS/1/1062-01-01. This work was supported by grants of The King Abdullah University of Science and Technology (KAUST) International Program OCRF-2014-CRG4 to Prof Heribert Hirt and Dr. Martin Crespi. Dr Martin Crespi benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Publisher
Public Library of Science (PLoS)Journal
PLOS PathogensPubMed ID
32302366Additional Links
https://dx.plos.org/10.1371/journal.ppat.1008401ae974a485f413a2113503eed53cd6c53
10.1371/journal.ppat.1008401
Scopus Count
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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