Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice
Type
ArticleAuthors
Shao, JingxiaHaider, Imran

Xiong, Lizhong
Zhu, Xiaoyi
Hussain, Rana Muhammad Fraz
Övernäs, Elin
Meijer, Annemarie H.
Zhang, Gaisheng
Wang, Mei
Bouwmeester, Harro J.
Ouwerkerk, Pieter B. F.
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionKing Abdullah University of Science and Technology (KAUST), The BioActives Lab,
Date
2018-07-20Permanent link to this record
http://hdl.handle.net/10754/630761
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Show full item recordAbstract
The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.Citation
Shao J, Haider I, Xiong L, Zhu X, Hussain RMF, et al. (2018) Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice. PLOS ONE 13: e0199248. Available: http://dx.doi.org/10.1371/journal.pone.0199248.Sponsors
We thank all the contributing members of the Rice Research Group of the IBL, Leiden University. This work was supported by funding to JS from China Scholarship Council (CSC, 2007U27088) and National Natural Science Foundation of China (31770345). This work was supported by the Higher Education Commission (HEC) of Pakistan and the King Abdullah University of Science and Technology (KAUST) to IH. MW and PBFO were supported by the KNAW Program Scientific Alliance (04-PSA-BD-04). AHM was supported by TF-STRESS (QLK3-CT-2000-00328). PBFO was also supported by CEDROME (INCO-CT-2005-015468).Publisher
Public Library of Science (PLoS)Journal
PLOS ONEAdditional Links
http://europepmc.org/articles/pmc6054374?pdf=renderae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0199248
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