Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice
Hussain, Rana Muhammad Fraz
Meijer, Annemarie H.
Bouwmeester, Harro J.
Ouwerkerk, Pieter B. F.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
King Abdullah University of Science and Technology (KAUST), The BioActives Lab,
Permanent link to this recordhttp://hdl.handle.net/10754/630761
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AbstractThe homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.
CitationShao J, Haider I, Xiong L, Zhu X, Hussain RMF, et al. (2018) Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice. PLOS ONE 13: e0199248. Available: http://dx.doi.org/10.1371/journal.pone.0199248.
SponsorsWe thank all the contributing members of the Rice Research Group of the IBL, Leiden University. This work was supported by funding to JS from China Scholarship Council (CSC, 2007U27088) and National Natural Science Foundation of China (31770345). This work was supported by the Higher Education Commission (HEC) of Pakistan and the King Abdullah University of Science and Technology (KAUST) to IH. MW and PBFO were supported by the KNAW Program Scientific Alliance (04-PSA-BD-04). AHM was supported by TF-STRESS (QLK3-CT-2000-00328). PBFO was also supported by CEDROME (INCO-CT-2005-015468).
PublisherPublic Library of Science (PLoS)
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