Molecular analyses of protists in long-term observation programmes—current status and future perspectives
Kooistra, Wiebe H C F
Moran, Xose Anxelu G.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Red Sea Research Center (RSRC)
Online Publication Date2018-09-15
Print Publication Date2018-09-01
Permanent link to this recordhttp://hdl.handle.net/10754/630530
MetadataShow full item record
AbstractProtists (microbial eukaryotes) are diverse, major components of marine ecosystems, and are fundamental to ecosystem services. In the last 10 years, molecular studies have highlighted substantial novel diversity in marine systems including sequences with no taxonomic context. At the same time, many known protists remain without a DNA identity. Since the majority of pelagic protists are too small to identify by light microscopy, most are neither comprehensively or regularly taken into account, particularly in Long-term Ecological Research Sites. This potentially undermines the quality of research and the accuracy of predictions about biological species shifts in a changing environment. The ICES Working Group for Phytoplankton and Microbial Ecology conducted a questionnaire survey in 2013–2014 on methods and identification of protists using molecular methods plus a literature review of protist molecular diversity studies. The results revealed an increased use of high-throughput sequencing methods and a recognition that sequence data enhance the overall datasets on protist species composition. However, we found only a few long-term molecular studies and noticed a lack of integration between microscopic and molecular methods. Here, we discuss and put forward recommendations to improve and make molecular methods more accessible to Long-term Ecological Research Site investigators.
CitationStern R, Kraberg A, Bresnan E, Kooistra WHCF, Lovejoy C, et al. (2018) Molecular analyses of protists in long-term observation programmes—current status and future perspectives. Journal of Plankton Research 40: 519–536. Available: http://dx.doi.org/10.1093/plankt/fby035.
SponsorsWe wish to thank all participants in this survey, the ICES Working Group for Phytoplankton and Microbial Ecology and a special thanks to Jule Carstens who helped gather data together.
PublisherOxford University Press (OUP)
JournalJournal of Plankton Research