In vivo reprogramming of wound-resident cells generates skin epithelial tissue
Type
ArticleAuthors
Kurita, MasakazuAraoka, Toshikazu
Hishida, Tomoaki
O’Keefe, David D.
Takahashi, Yuta
Sakamoto, Akihisa
Sakurai, Masahiro
Suzuki,Keiichiro
Wu, Jun
Yamamoto, Mako
Hernandez-Benitez, Reyna
Ocampo, Alejandro
Reddy, Pradeep
Shokhirev, Maxim Nikolaievich
Magistretti, Pierre J.

Núñez Delicado, Estrella
Eto, Hitomi
Harii, Kiyonori
Izpisua Belmonte, Juan Carlos
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Date
2018-09-05Online Publication Date
2018-09-05Print Publication Date
2018-09Permanent link to this record
http://hdl.handle.net/10754/630520
Metadata
Show full item recordAbstract
Large cutaneous ulcers are, in severe cases, life threatening1,2. As the global population ages, non-healing ulcers are becoming increasingly common1,2. Treatment currently requires the transplantation of pre-existing epithelial components, such as skin grafts, or therapy using cultured cells2. Here we develop alternative supplies of epidermal coverage for the treatment of these kinds of wounds. We generated expandable epithelial tissues using in vivo reprogramming of wound-resident mesenchymal cells. Transduction of four transcription factors that specify the skin-cell lineage enabled efficient and rapid de novo epithelialization from the surface of cutaneous ulcers in mice. Our findings may provide a new therapeutic avenue for treating skin wounds and could be extended to other disease situations in which tissue homeostasis and repair are impaired.Citation
Kurita M, Araoka T, Hishida T, O’Keefe DD, Takahashi Y, et al. (2018) In vivo reprogramming of wound-resident cells generates skin epithelial tissue. Nature 561: 243–247. Available: http://dx.doi.org/10.1038/s41586-018-0477-4.Sponsors
This work was supported by MEXT KAKENHI Grant numbers JP26293381(Grant-in-Aid for Scientific Research (B) to M.K.), JP23689073 (Grant-in-Aid for Young Scientists (A) to M.K.), JP21689046 (Grant-in-Aid for Young Scientists (A) to M.K.), Kyorin University research promotion award to M.K. (2013), JSPS Overseas Research Fellowships (2015–17) to M.K., and the Uehara Memorial Foundation Research Fellowship for Research Abroad (2017–18) to M.K. M.K. thanks H. Green for support materials. T.H. thanks F. Sugiyama for support materials. M.N.S. is supported by NIH-NCI CCSG: P30 014195 and The Leona M. and Harry B. Helmsley Charitable Trust. Work in the laboratory of J.C.I.B. was supported by the G. Harold and Leila Y. Mathers Charitable Foundation, The Leona M. and Harry B. Helmsley Charitable Trust, The Moxie Foundation, The Evergreen Foundation, Fundacion Dr. Pedro Guillen and Universidad Católica San Antonio de Murcia (UCAM).Publisher
Springer NatureJournal
NatureAdditional Links
https://www.nature.com/articles/s41586-018-0477-4ae974a485f413a2113503eed53cd6c53
10.1038/s41586-018-0477-4