• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    Infrared spectroscopy reveals multi-step multi-timescale photoactivation in the photoconvertible protein archetype dronpa

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Infrared sprectroscopy.pdf
    Size:
    604.8Kb
    Format:
    PDF
    Description:
    Accepted manuscript
    Download
    Type
    Article
    Authors
    Laptenok, Siarhei cc
    Gil, Agnieszka A.
    Hall, Christopher R.
    Lukacs, Andras
    Iuliano, James N.
    Jones, Garth A.
    Greetham, Gregory M.
    Donaldson, Paul
    Miyawaki, Atsushi
    Tonge, Peter J.
    Meech, Stephen R.
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2018-06-11
    Online Publication Date
    2018-06-11
    Print Publication Date
    2018-08
    Permanent link to this record
    http://hdl.handle.net/10754/630489
    
    Metadata
    Show full item record
    Abstract
    Photochromic fluorescent proteins play key roles in super-resolution microscopy and optogenetics. The light-driven structural changes that modulate the fluorescence involve both trans-to-cis isomerization and proton transfer. The mechanism, timescale and relative contribution of chromophore and protein dynamics are currently not well understood. Here, the mechanism of off-to-on-state switching in dronpa is studied using femtosecond-to-millisecond time-resolved infrared spectroscopy and isotope labelling. Chromophore and protein dynamics are shown to occur on multiple timescales, from picoseconds to hundreds of microseconds. Following excitation of the trans chromophore, a ground-state primary product is formed within picoseconds. Surprisingly, the characteristic vibrational spectrum of the neutral cis isomer appears only after several tens of nanoseconds. Further fluctuations in protein structure around the neutral cis chromophore are required to form a new intermediate, which promotes the final proton-transfer reaction. These data illustrate the interplay between chromophore dynamics and the protein environment underlying fluorescent protein photochromism.
    Citation
    Laptenok SP, Gil AA, Hall CR, Lukacs A, Iuliano JN, et al. (2018) Infrared spectroscopy reveals multi-step multi-timescale photoactivation in the photoconvertible protein archetype dronpa. Nature Chemistry 10: 845–852. Available: http://dx.doi.org/10.1038/s41557-018-0073-0.
    Sponsors
    S.R.M. acknowledges EPSRC for financial support (EP/N033647/1 and EP/M001997/1). P.J.T. acknowledges NSF for financial support (CHE-1223819). A.M. acknowledges the Japan Ministry of Education, Culture, Sports, Science and Technology Grant-in-aid for Scientific research on Innovative Areas: Resonance Bio. The authors acknowledge STFC for access to the Central Laser Facility. Calculations were performed on the High Performance Computing Cluster at the University of East Anglia.
    Publisher
    Springer Nature
    Journal
    Nature Chemistry
    DOI
    10.1038/s41557-018-0073-0
    Additional Links
    http://link.springer.com/article/10.1038/s41557-018-0073-0
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41557-018-0073-0
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division

    entitlement

     
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.