• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    A uniform bacterial growth potential assay for different water types

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Farhat-2018-A_uniform_bacterial_growth_potential-(accepted_version).pdf
    Size:
    945.8Kb
    Format:
    PDF
    Description:
    Accepted manuscript
    Download
    Thumbnail
    Name:
    1-s2.0-S0043135418304548-mmc1.docx
    Size:
    23.46Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplementary Material
    Download
    Type
    Article
    Authors
    Siddiqui, Amber cc
    Hammes, Frederik
    Prest, Emmanuelle
    Vrouwenvelder, Johannes S. cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Environmental Science and Engineering Program
    Water Desalination and Reuse Research Center (WDRC)
    Date
    2018-06-06
    Online Publication Date
    2018-06-06
    Print Publication Date
    2018-10
    Embargo End Date
    2020-06-06
    Permanent link to this record
    http://hdl.handle.net/10754/630470
    
    Metadata
    Show full item record
    Abstract
    The bacterial growth potential is important to understand and manage bacterial regrowth-related water quality concerns. Bacterial growth potential depends on growth promoting/limiting compounds, therefore, nutrient availability is the key factor governing bacterial growth potential. Selecting proper tools for bacterial growth measurement is essential for routine implementation of the growth potential measurement. This study proposes a growth potential assay that is universal and can be used for different water types and soil extract without restrictions of pure culture or cultivability of the bacterial strain. The proposed assay measures the sample bacterial growth potential by using the indigenous community as inocula. Flow cytometry (FCM) and adenosine tri-phosphate (ATP) were used to evaluate the growth potential of six different microbial communities indigenous to the sample being analyzed, with increasing carbon concentrations. Bottled mineral water, non-chlorinated tap water, seawater, river water, wastewater effluent and a soil organic carbon extract were analyzed. Results showed that indigenous bacterial communities followed normal batch growth kinetics when grown on naturally present organic carbon. Indigenous bacterial growth could detect spiked organic carbon concentrations as low as 10 μg/L. The indigenous community in all samples responded proportionally to the increase in acetate-carbon and proportional growth could be measured with both FCM and ATP. Bacterial growth was proportional to the carbon concentration but not the same proportion factor for the different water samples tested. The effect of inoculating the same water with different indigenous microbial communities on the growth potential was also examined. The FCM results showed that the highest increase in total bacterial cell concentration was obtained with bacteria indigenous to the water sample. The growth potential assay using indigenous bacterial community revealed consistent results of bacterial growth in all the different samples tested and therefore providing a fast, more stable, and accurate approach for monitoring the biological stability of waters compared to the previously developed assays. The growth potential assay can be used to aid in detecting growth limitations by compounds other than organic carbon.
    Citation
    Farhat N, Hammes F, Prest E, Vrouwenvelder J (2018) A uniform bacterial growth potential assay for different water types. Water Research 142: 227–235. Available: http://dx.doi.org/10.1016/j.watres.2018.06.010.
    Sponsors
    The authors acknowledge the financial support of KAUST, Evides, and Eawag. The authors acknowledge Stefan Koetzsch, Joao Mimoso, and Hans-Ulrich Weilenmann for assistance with experiments and data analysis.
    Publisher
    Elsevier BV
    Journal
    Water Research
    DOI
    10.1016/j.watres.2018.06.010
    Additional Links
    http://www.sciencedirect.com/science/article/pii/S0043135418304548
    https://www.dora.lib4ri.ch/eawag/islandora/object/eawag%3A17236/datastream/PDF2/Farhat-2018-A_uniform_bacterial_growth_potential-%28accepted_version%29.pdf
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.watres.2018.06.010
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)

    entitlement

     
    DSpace software copyright © 2002-2023  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.