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dc.contributor.authorYates, Steven
dc.contributor.authorMikaberidze, Alexey
dc.contributor.authorKrattinger, Simon G.
dc.contributor.authorAbrouk, Michael
dc.contributor.authorHund, Andreas
dc.contributor.authorYu, Kang
dc.contributor.authorStuder, Bruno
dc.contributor.authorFouche, Simone
dc.contributor.authorMeile, Lukas
dc.contributor.authorPereira, Danilo
dc.contributor.authorKaristo, Petteri
dc.contributor.authorMcDonald, Bruce
dc.date.accessioned2018-12-31T08:58:41Z
dc.date.available2018-12-31T08:58:41Z
dc.date.issued2018-12-21
dc.identifier.citationYates S, Mikaberidze A, Krattinger S, Abrouk M, Hund A, et al. (2018) Precision phenotyping reveals novel loci for quantitative resistance to septoria tritici blotch in European winter wheat. Available: http://dx.doi.org/10.1101/502260.
dc.identifier.doi10.1101/502260
dc.identifier.urihttp://hdl.handle.net/10754/630380
dc.description.abstractAccurate, high-throughput phenotyping for quantitative traits is the limiting factor for progress in plant breeding. We developed automated image analysis to measure quantitative resistance to septoria tritici blotch (STB), a globally important wheat disease, enabling identification of small chromosome intervals containing plausible candidate genes for STB resistance. 335 winter wheat cultivars were included in a replicated field experiment that experienced natural epidemic development by a highly diverse but fungicide-resistant pathogen population. More than 5.4 million automatically generated phenotypes were associated with 13,648 SNP markers to perform a GWAS. We identified 26 chromosome intervals explaining 1.9-10.6% of the variance associated with four resistance traits. Seventeen of the intervals were less than 5 Mbp in size and encoded only 173 genes, including many genes associated with disease resistance. Five intervals contained four or fewer genes, providing high priority targets for functional validation. Ten chromosome intervals were not previously associated with STB resistance. Our experiment illustrates how high-throughput automated phenotyping can accelerate breeding for quantitative disease resistance. The SNP markers associated with these chromosome intervals can be used to recombine different forms of quantitative STB resistance that are likely to be more durable than pyramids of major resistance genes.
dc.description.sponsorshipSTB research in BAM's lab was supported by the Swiss National Science Foundation (grants 155955, 134755, 104145 and 56874) and the ETH Zurich Research Commission (grants 12-03, 15-02). AM and PK were supported by the Swiss National Science Foundation through Ambizione grant PZ00P3_161453. H. Zellweger managed the wheat trial. Marion Roeder from IPK Gatersleben provided seeds and marker information for the GABI wheat panel.
dc.publisherCold Spring Harbor Laboratory
dc.relation.urlhttps://www.biorxiv.org/content/early/2018/12/20/502260
dc.rightsThe copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titlePrecision phenotyping reveals novel loci for quantitative resistance to septoria tritici blotch in European winter wheat
dc.typePreprint
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.eprint.versionPre-print
dc.contributor.institutionMolecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland
dc.contributor.institutionPlant Pathology, Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland
dc.contributor.institutionCrop Science, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland
kaust.personKrattinger, Simon G.
kaust.personAbrouk, Michael
refterms.dateFOA2018-12-31T12:11:29Z


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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.