ATP measurement in seawater reverse osmosis systems: Eliminating seawater matrix effects using a filtration-based method
Salinas-Rodriguez, Sergio G.
Mangal, Muhammad Nasir
Goueli, Said A.
Vrouwenvelder, Johannes S.
Schippers, Jan C.
Kennedy, Maria D.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
Online Publication Date2018-12-06
Print Publication Date2019-03
Permanent link to this recordhttp://hdl.handle.net/10754/630332
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AbstractA direct method for measuring adenosine-triphosphate (ATP) in seawater was developed recently, in which commercial reagents are added directly to seawater. However, calibration is required if seawater quality changes (such as changes in salinity, pH, Mg, Fe) as the seawater matrix interferes with ATP measurement. In this research, a 0.1 μm filtration process is introduced to eliminate such interferences. In addition, a filter rinsing step with sterilized artificial seawater is proposed to eliminate interference of free ATP. The ATP-filtration method is fast (<5 min), reproducible (VC = 7%), six times more sensitive than the direct ATP-method and correlates (R = 0.72, n = 100) with intact cell concentration. Microbial ATP concentration measured using the ATP-filtration method and the ATP-direct method were comparable. Microbial ATP measured along the treatment train of a full-scale seawater reverse osmosis (SWRO) plant decreased from 530 in the raw seawater to 10 ng-ATP/L after pre-treatment and to 0.5 ng-ATP/L in the SWRO permeate. The method was also applied to monitor bacterial growth potential (BGP) across the pre-treatment train of a (pilot) seawater desalination plant, where the removal of BGP through the media filtration and ultrafiltration was 44% and 7%, respectively.
CitationAbushaban A, Salinas-Rodriguez SG, Mangal MN, Mondal S, Goueli SA, et al. (2019) ATP measurement in seawater reverse osmosis systems: Eliminating seawater matrix effects using a filtration-based method. Desalination 453: 1–9. Available: http://dx.doi.org/10.1016/j.desal.2018.11.020.
SponsorsWe thank Loreen O. Villacorte for his contribution in this research. Also thanks to David Grasso for helping obtain access and collecting samples from the full-scale seawater desalination plant. Special thanks are due to Sophie Boettger, Almohanad Abusultan, Chidiebere Nnebuo and Maud Salvaresi for their assistance in the preliminary work of this research.
Except where otherwise noted, this item's license is described as This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).