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dc.contributor.authorJanáková, Eva
dc.contributor.authorJakobson, Irena
dc.contributor.authorPeusha, Hilma
dc.contributor.authorAbrouk, Michael
dc.contributor.authorŠkopová, Monika
dc.contributor.authorŠimková, Hana
dc.contributor.authorŠafář, Jan
dc.contributor.authorVrána, Jan
dc.contributor.authorDoležel, Jaroslav
dc.contributor.authorJärve, Kadri
dc.contributor.authorValárik, Miroslav
dc.date.accessioned2018-12-11T11:56:52Z
dc.date.available2018-12-11T11:56:52Z
dc.date.issued2018-12-07
dc.identifier.citationJanáková E, Jakobson I, Peusha H, Abrouk M, Škopová M, et al. (2018) Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene. Theoretical and Applied Genetics. Available: http://dx.doi.org/10.1007/s00122-018-3259-3.
dc.identifier.issn0040-5752
dc.identifier.issn1432-2242
dc.identifier.doi10.1007/s00122-018-3259-3
dc.identifier.urihttp://hdl.handle.net/10754/630263
dc.description.abstractA segment of Triticum militinae chromosome 7G harbors a gene(s) conferring powdery mildew resistance which is effective at both the seedling and the adult plant stages when transferred into bread wheat (T. aestivum). The introgressed segment replaces a piece of wheat chromosome arm 4AL. An analysis of segregating materials generated to positionally clone the gene highlighted that in a plant heterozygous for the introgression segment, only limited recombination occurs between the introgressed region and bread wheat 4A. Nevertheless, 75 genetic markers were successfully placed within the region, thereby confining the gene to a 0.012 cM window along the 4AL arm. In a background lacking the Ph1 locus, the localized rate of recombination was raised 33-fold, enabling the reduction in the length of the region containing the resistance gene to a 480 kbp stretch harboring 12 predicted genes. The substituted segment in the reference sequence of bread wheat cv. Chinese Spring is longer (640 kbp) and harbors 16 genes. A comparison of the segments’ sequences revealed a high degree of divergence with respect to both their gene content and nucleotide sequence. Of the 12 T. militinae genes, only four have a homolog in cv. Chinese Spring. Possible candidate genes for the resistance have been identified based on function predicted from their sequence.
dc.description.sponsorshipThe technical assistance of Marie Seifertová, Radka Tušková, Eva Jahnová and Helena Tvardíková is highly appreciated. This research was financially supported by the Czech Republic Ministry of Education, Youth and Sports (Award LO1204 from the National Program of Sustainability I), by the Czech Science Foundation (Award 18-11688S), the Czech Republic Ministry of Agriculture (Award QK1710302) and by an IUT 193 Grant from the Estonian Ministry of Education and Research. The authors thank the International Wheat Genome Sequencing Consortium (IWGSC) for pre-publication access to IWGSC RefSeq v1.0, the National BioResource Centre (Kyoto, Japan) for providing the CS aneuploid stocks and S. Reader (John Innes Centre, Norwich, UK) for the gift of grain of the ph1b mutant. Access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum provided under the program “Projects of Large Research, Development and Innovation Infrastructures” (CESNET LM2015042) is greatly appreciated.
dc.publisherSpringer Nature
dc.relation.urlhttps://link.springer.com/article/10.1007%2Fs00122-018-3259-3
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleDivergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalTheoretical and Applied Genetics
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic
dc.contributor.institutionDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia
dc.contributor.institutionLimagrain Central Europe Cereals, Bedihošť, Czech Republic
kaust.personAbrouk, Michael
refterms.dateFOA2018-12-11T12:01:51Z
dc.date.published-online2018-12-07
dc.date.published-print2019-04


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.