• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    Drinking Water Microbial Communities

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Dissertation_Final.pdf
    Size:
    21.50Mb
    Format:
    PDF
    Description:
    PhD Dissertation
    Download
    Type
    Dissertation
    Authors
    El-Chakhtoura, Joline cc
    Advisors
    Vrouwenvelder, Johannes S. cc
    Committee members
    Saikaly, Pascal cc
    Loosdrecht, Mark van
    Stenchikov, Georgiy L. cc
    Program
    Environmental Science and Engineering
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2018-11
    Embargo End Date
    2019-12-09
    Permanent link to this record
    http://hdl.handle.net/10754/630222
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2019-12-09.
    Abstract
    Water crises are predicted to be amongst the risks of highest concern for the next ten years, due to availability, accessibility, quality and management issues. Knowledge of the microbial communities indigenous to drinking water is essential for treatment and distribution process control, risk assessment and infrastructure design. Drinking water distribution systems (DWDSs) ideally should deliver to the consumer water of the same microbial quality as that leaving a treatment plant (“biologically stable” according to WHO). At the start of this Ph.D. program water microbiology comprised conventional culturedependent methods, and no studies were available on microbial communities from source to tap. A method combining 16S rRNA gene pyrosequencing with flow cytometry was developed to accurately detect, characterize, and enumerate the microorganisms found in a water sample. Studies were conducted in seven fullscale Dutch DWDSs which transport low-AOC water without disinfectant residuals, produced from fresh water applying conventional treatment. Full-scale studies were also conducted at the desalination plant and DWDS of KAUST, Saudi Arabia where drinking water is produced from seawater applying RO membrane treatment and then transported with chlorine residual. Furthermore, biological stability was evaluated in a wastewater reuse application in the Netherlands. When low-AOC water was distributed without disinfectant residuals, greater bacterial richness was detected in the networks, however, temporal and spatial variations in the bacterial community were insignificant and a substantial fraction of the microbiome was still shared between the treated and transported water. This shared fraction was lower in the system transporting water with chlorine residual, where the eukaryotic community changed with residence time. The core microbiome was characterized and dominant members varied between the two systems. Biofilm and deposit-associated communities were found to drive tap water microbiology regardless of water source and treatment scheme. Network flushing was found to be a simple method to assess water microbiology. Biological stability was not associated with safety. The biological stability concept needs to be revised and quantified. Further research is needed to understand microbial functions and processes, how water communities affect the human microbiome, and what the “drinking” water microbiome is like in undeveloped countries.
    Citation
    El-Chakhtoura, J. (2018). Drinking Water Microbial Communities. KAUST Research Repository. https://doi.org/10.25781/KAUST-L89LT
    Sponsors
    The research presented in this doctoral dissertation was financially supported by and conducted in collaboration with Delft University of Technology (TU Delft) and Evides Waterbedrijf in the Netherlands.
    DOI
    10.25781/KAUST-L89LT
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-L89LT
    Scopus Count
    Collections
    Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Dissertations

    entitlement

     
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.