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dc.contributor.authorMalara, N.
dc.contributor.authorGentile, F.
dc.contributor.authorCoppedè, N.
dc.contributor.authorColuccio, M. L.
dc.contributor.authorCandeloro, P.
dc.contributor.authorPerozziello, G.
dc.contributor.authorFerrara, L.
dc.contributor.authorGiannetto, M.
dc.contributor.authorCareri, M.
dc.contributor.authorCastellini, A.
dc.contributor.authorMignogna, C.
dc.contributor.authorPresta, I.
dc.contributor.authorPirrone, C. K.
dc.contributor.authorMaisano, D.
dc.contributor.authorDonato, A.
dc.contributor.authorDonato, G.
dc.contributor.authorGreco, M.
dc.contributor.authorScumaci, D.
dc.contributor.authorCuda, Giovanni
dc.contributor.authorCasale, F.
dc.contributor.authorFerraro, E.
dc.contributor.authorBonacci, S.
dc.contributor.authorTrunzo, V.
dc.contributor.authorMollace, V.
dc.contributor.authorOnesto, V.
dc.contributor.authorMajewska, R.
dc.contributor.authorAmato, F.
dc.contributor.authorRenne, M.
dc.contributor.authorInnaro, N.
dc.contributor.authorSena, G.
dc.contributor.authorSacco, R.
dc.contributor.authorGivigliano, F.
dc.contributor.authorVoci, C.
dc.contributor.authorVolpentesta, G.
dc.contributor.authorGuzzi, G.
dc.contributor.authorLavano, A.
dc.contributor.authorScali, E.
dc.contributor.authorBottoni, U.
dc.contributor.authorDi Fabrizio, Enzo M.
dc.date.accessioned2018-12-05T08:06:11Z
dc.date.available2018-12-05T08:06:11Z
dc.date.issued2018-11-19
dc.identifier.citationMalara N, Gentile F, Coppedè N, Coluccio ML, Candeloro P, et al. (2018) Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour. npj Precision Oncology 2. Available: http://dx.doi.org/10.1038/s41698-018-0069-7.
dc.identifier.issn2397-768X
dc.identifier.doi10.1038/s41698-018-0069-7
dc.identifier.urihttp://hdl.handle.net/10754/630183
dc.description.abstractSecretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual's diagnosis and/or help clarify risk in healthy populations.
dc.description.sponsorshipThis paper is dedicated to the memory of our colleague Prof. Ugo Bottoni. This work has been partially funded from the Italian Ministry of Health (Project n. GR-2010–2320665) and (Project n. GR-2010–2311677) by the project Interregional Research Centre for Food Safety & Health (IRC_FSH) (cod. PON a3–00359), granted to the Department of Health Science, University of Magna Graecia. The authors also acknowledge financial support from KAUST funding through CRG5 project OSR award #: OCRF-2016-CRG5. NC acknowledges Nicola Zambelli and Giacomo Benassi for technical support. NM acknowledges Prof. Bruno Silvestrini and Gianluca Rotta for their precious scientific support and Nastassia C. Garo for her technical assistance. The authors would like to thank the patients and all health professionals involved at the University of 'Magna Graecia' and Mater Domini Hospital and ANASTE nursing home.
dc.publisherSpringer Nature
dc.relation.urlhttps://www.nature.com/articles/s41698-018-0069-7
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0
dc.titleSuperhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour
dc.typeArticle
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalnpj Precision Oncology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University Magna Graecia, 88100 Catanzaro, Italy.
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University Federico II, 80125 Naples, Italy.
dc.contributor.institutionInstitute of Materials for Electronics and Magnetism IMEM CNR Parco Area delle Scienze, 43124 Parma, Italy.
dc.contributor.institutionCentre of Medical Physics and Biomedical Engineering, Medical University of Vienna, 1090 Vienna, Austria.
dc.contributor.institutionDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
dc.contributor.institutionDepartment of Health Science, University Magna Graecia, 88100 Catanzaro, Italy.
dc.contributor.institutionDepartment of Clinical and Biological Sciences, University of Torino, Torino, Italy.
dc.contributor.institutionSchool of Biological Sciences, North-West University, Potchefstroom, 2520 South Africa.
dc.contributor.institutionDepartment of Surgical and Medical Science University Magna Graecia, 88100 Catanzaro, Italy.
kaust.personDi Fabrizio, Enzo M.
kaust.grant.numberOCRF-2016-CRG5
refterms.dateFOA2018-12-05T08:35:42Z
dc.date.published-online2018-11-19
dc.date.published-print2018-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.