TGF-b2, catalase activity, H2O2 output and metastatic potential of diverse types of tumour
Type
PreprintKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionComputational Bioscience Research Center (CBRC)
Pathogen Genomics Laboratory
Date
2018-11-14Permanent link to this record
http://hdl.handle.net/10754/629953
Metadata
Show full item recordAbstract
Theileria annulata is a protozoan parasite that infects and transforms bovine macrophages causing a myeloid-leukaemia-like disease called tropical theileriosis. TGF-b2 is highly expressed in many cancer cells and is significantly increased in Theileria-transformed macrophages, as are levels of Reactive Oxygen Species (ROS), notably H2O2. Here, we describe the interplay between TGF-b2 and ROS in cellular transformation. We show that TGF-b2 drives expression of catalase to reduce the amount of H2O2 produced by T. annulata-transformed bovine macrophages, as well as by human lung (A549) and colon cancer (HT-29) cell lines. Theileria-transformed macrophages attenuated for dissemination express less catalase and produce more H2O2, but regain both virulent migratory and matrigel traversal phenotypes when stimulated with TGF-b2, or catalase that reduce H2O2 output. Increased H2O2 output therefore, underpins the aggressive dissemination phenotype of diverse tumour cell types, but in contrast, too much H2O2 can dampen dissemination.Citation
Haidar M, Metheni M, Batteux F, Langsley G (2018) TGF-b2, catalase activity, H2O2 output and metastatic potential of diverse types of tumour. Available: http://dx.doi.org/10.1101/467191.Sponsors
We thank Arnab Pain for fruitful discussion and input when writing the manuscript. MH was supported by a PhD CNR fellowship from the Lebanese government. GL acknowledges support from ANR grant (11 BSV3 01602), Labex ParaFrap (ANR-11-LABX-0024) and core support from INSERM and the CNRS.Publisher
Cold Spring Harbor LaboratoryDOI
10.1101/467191Additional Links
https://www.biorxiv.org/content/early/2018/11/14/467191ae974a485f413a2113503eed53cd6c53
10.1101/467191